You Searched For: Cellular+Assays

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Water, meets analytical specification of Ph. Eur., USP for injection (WFI), cell culture reagent, sterile, Corning®

Supplier: Corning

Description: Cell culture grade water is high-quality water suitable for use as a solvent in the preparation of cell culture media and other reagents for sensitive applications. Our bioprocess container selections are suitable for large-scale applications such as cleaning and upstream and downstream reagent preparation. Cell culture grade water goes through a wide array of testing which includes testing to the chemical and physical properties found in the USP and EP monographs for sterile Water for Injection (WFI).

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VWR®, Sealing Film for Biological Cultures

Supplier: VWR Collection

Description: Rayon film with medical grade adhesive. Hydrophobic, porous film in sheeted roll format for sealing tissue culture plates, bio-blocks, and 96-well plates where gas exchange is necessary for cell or bacterial growth.

L(+)-Glutamine solution 200 mM (100x) in water + 8.5 g/L NaCl cell culture reagent, Corning®

Catalog Number: (MDTC25-005-CV)

Supplier: Corning

Description: L-glutamine is an essential amino acid and a key component of culture media, serving as a major energy source for propagating cells. It is very stable as a dry powder and as a frozen solution but degrades rapidly in liquid media or stock solutions, producing toxic compounds. Optimal cell performance usually requires supplementation of the media with L-glutamine prior to use. This formulation is prepared in cell culture grade water.

UOM: 1 * 6 items

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L(+)-Glutamine solution 200 mM in water (29.20 mg/ml) cell culture reagent, Corning®

Catalog Number: (25-005-CI)

Supplier: Corning

UOM: 1 * 6 items

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Vertical rotator, 360°, PTR-25

Catalog Number: (444-0932)

Supplier: GRANT INSTRUMENTS

Description: Compact rotator with easy to use controls, provides thorough end over end mixing of samples in tubes up to 50 ml. Applications include hybridisation reactions, cell growth, soft extraction, binding reactions, preventing blood coagulation and homogenisation of biological components in solution. The reliable motor produces regulated rotation throughout the speed range.

UOM: 1 * 1 items

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HEPES (2-[4-(2-hydroxyethyl)-1-piperazinyl]ethanesulphonic acid) ≥99.5%

Catalog Number: (441476L)

Supplier: VWR Chemicals

Description: Hepes is a widely used buffer in biological studies. In cell culture media, it is employed as a substitute for the bicarbonate buffer at a concentration of 25 mM or as a supplement to the bicarbonate buffer (concentration 10 to 15 mM).

UOM: 1 * 1 kg

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Mixer mill, CryoMill

Catalog Number: (412-0232)

Supplier: RETSCH

Description: The CryoMill has been specially designed for cryogenic grinding and it is ideal for plastics, temperature sensitive materials or samples with volatile components. Applications include cell disruption, cryogenic, wet and dry grinding of hard, medium hard, soft, brittle, elastic or fibrous materials. The grinding jar is continually cooled with liquid nitrogen at –196 °C from the integrated cooling system before and during the grinding process.

UOM: 1 * 1 items

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Tangential flow systems, Vivaflow® 50 and 200

Supplier: Sartorius

Description: Vivaflow® 50 and 200 devices are ready to use crossflow cassettes for filtration and concentration of samples from 100 to 3000 ml or 500 to 5000 ml. The membrane surface per device is 50 cm² or 200 cm² respectively. The devices come with all the necessary accessories for running with a laboratory pump and a size 16 pump head. For speeding up the concentration and increasing the volume throughput, up to six Vivaflow® 50 cassettes can be connected together or two Vivaflow® 200 can be connected together. Optimally suited for applications like cell culture supernatant concentration, virus concentration and water concentration this economical product offers a standard of ease of use, reliability and flexibility.

Vortex mixers, Disruptor Genie®

Supplier: SCIENTIFIC INDUSTRIES ELMIS

Description: An affordable alternative to ultrasonic disruptors. Units provide multidirectional action which simultaneously agitates and vortexes at high speed, increasing disruption frequency. Ideal for rapid disruption of yeast cells, bacteria, plant and animal tissue, pellet resuspensions and glass bead procedures.

VWR®, Microplate Shaker, High Speed

Catalog Number: (444-7016)

Supplier: VWR Collection

Description: Ideal for molecular biology applications, mechanical and chemical cell lysis, mixing tissue samples, mixing cytogenetic suspensions, and vortexing cell suspensions. Shaker is also ideal for emulsification of bi-polar viscous fluids and for mixing fluids in small diameter tubes. Specifically designed to shake and/or vortex microplates in timed or continuous modes. Shaker features programmable timed mode from 1 s to 160 hours. Speed and time are easily adjusted with the up/down arrows on the front panel.

UOM: 1 * 1 items

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VWR®, Breatheable Rayon Film Seals for Biological Cultures

Supplier: VWR Collection

Description: A 139,7 µm thick hydrophobic porous film with medical grade adhesive for tissue culture plates, bio-blocks, and 96-well plates where gas exchange is necessary for cell or bacterial growth. These rayon films minimise cross-contamination, spillage and evaporation. They allow uniform air and CO₂ exchange for all wells, unlike plate lids which favour exchange for wells near plate edges. Sterile product is packed in tamper evident bags of 25.

E.Z.N.A.® Endo-Free plasmid mini kit II

Supplier: OMEGA BIOTEK

Description: Plasmid isolated with traditional purification procedures normally contain high levels of endotoxins (also known as lipopolysaccharides or LPS) that can significantly interfere with transfection experiments downstream. The E.Z.N.A.® Endo-Free plasmid mini kit II integrates an efficient endotoxin removal step into the plasmid purification procedure to produce high-quality transfection grade (<0,1 EU/µg) plasmid for efficient transfection. The bacterial cells are lysed using the alkaline-SDS lysis method. The cleared cell lysate is then treated with ETR reagent to efficiently remove the endotoxins. After adjusting the binding condition, the cell lysate is applied into the HiBind® DNA column and purified DNA is eluted from the column membrane.

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