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Supplier: BIOWEST

Description: Minimum Essential Medium (MEM) with Earle's Balanced Salts is a modification of Eagle's earlier medium, Basal Medium Eagle (BME), containing higher concentrations of the essential nutrients. These media have demonstrated the ability to support a variety of normal and transformed cells in culture and contain Earle's Balanced Salts, which make them suitable for use in atmospheres charged with CO₂ gas.

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Supplier: BIOWEST

Description: FreeAdd 1X is a fully chemically defined substitute for animal serum. It provides the necessary nutritional support for cell growth, development and expression. It has been tested on a variety of cells and can be used in combination with basal medium to generate equal or even better cell culture performance.

Supplier: BIOWEST

Description: Human serum 'off-the-clot' is processed from human blood that has been coagulation-induced. It is collected from volunteer donors.

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Supplier: BIOWEST

Description: Newborn Calf Serum (NBCS) originates from 3 to 10 day old calves. It is a high quality, cost effective replacement for Foetal Bovine Serum (FBS) and contains a somewhat higher proportion of proteins and immunoglobulin, but fewer growth factors. With proper cell adaptation, it has been shown to perform very well in cell culture applications where routine cell growth and cell culture maintenance is the objective.

Supplier: BIOWEST

Description: Trypsin is a porcine pancreas-derived enzyme that is commonly used for the dissociation and disaggregation of anchorage-dependent mammalian cells and tissues. The concentration of trypsin necessary to dislodge the cells from their substrate depends on the sensitivity of the cells.

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Supplier: BIOWEST

Description: Donkey serum may be used as a blocking agent and controls in immunoassays. For immunohistochemistry and immunocytochemistry, donkey serum was used between 5 to 20% as a blocking agent in various studies.

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Catalog Number: (L0014-100)

Supplier: BIOWEST

Description: Glutamine penicillin streptamycin acts against gram-negative and gram-positive bacteria.

UOM: 1 * 100 mL

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Supplier: BIOWEST

Description: Ham's F12 was originally developed for the serum-free clonal growth of Chinese Hamster Ovary (CHO) cells, lung cells and mouse L-cells. It is frequently used with dialysed serum, hormones, selenium and other supplements for serum-free cultures. It is the medium of choice for supporting the growth of cells of rodent origin, particularly rabbit and rat and has proved to be an excellent cloning medium for the culture of myeloma and hybrid cells.

Catalog Number: (S2600-500)

Supplier: BIOWEST

Description: Plasma provides a broad spectrum of macromolecules, carrier proteins for lipoid substances and trace elements, attachment and spreading factors, low molecular weight nutrients, and hormones and growth factors that promote cell growth. It is used as a component of bioassays, immunoassays or enzyme assays.

UOM: 1 * 500 mL

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Supplier: BIOWEST

Description: Bovine serum albumin (BSA) is commonly used in cell culture protocols, particularly where protein supplementation is necessary and the other components of serum are unwanted. In cell culture, its main role is as a carrier of small molecules. Because of its negative charge, BSA binds water, salts, fatty acids, vitamins and hormones, then carries these bound components between tissues and cells. The binding capacity also makes BSA an effective scavenger to remove toxic substances, including pyrogens, from the medium.

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Supplier: BIOWEST

Description: Human plasma is processed from human blood that has been coagulation-induced. It is collected from volunteer donors.

Supplier: BIOWEST

Description: Bovine serum albumin (BSA) is commonly used in cell culture protocols, particularly where protein supplementation is necessary and the other components of serum are unwanted. In cell culture, its main role is as a carrier of small molecules. Because of its negative charge, BSA binds water, salts, fatty acids, vitamins and hormones, then carries these bound components between tissues and cells. The binding capacity also makes BSA an effective scavenger to remove toxic substances, including pyrogens, from the medium.

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Supplier: BIOWEST

Description: McCoy’s media were originally formulated for growth and support of lymphocytes. This final modification produced a medium identical to RPMI 1629. McCoy's 5A media supports the indefinite proliferation of Walker 256 carcinoma cells. In addition, it is excellent for the propagation of leukocytes, biopsy tissues, a broad range of human and rat normal or transformed cell types, the most current primary and continuous cell lines.

Supplier: BIOWEST

Description: Dog serum is the liquid fraction of whole blood that is collected after the blood is allowed to clot; it is the blood plasma with the fibrinogens removed. Serum includes all proteins not used in blood clotting (coagulation) and all the electrolytes, antibodies, antigens, hormones, and any exogenous substances. Dog serum is used as a blocking agent in immunoassays and controls.

Catalog Number: (P4030-250MG)

Supplier: BIOWEST

Description: Amphotericin B is used for the prevention or elimination of fungal contaminants in cell culture.

UOM: 1 * 250 mg

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Supplier: BIOWEST

Description: Antibiotic-antimycotic used in tissue culture to prevent growth of bacteria (gram positive and gram negative), yeast and fungi. Penicillin acts by inhibiting bacterial cell wall synthesis.

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