You Searched For: qPCR\\\/RT-qPCR+Enzymes+and+Kits

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Accessories for Hach colour test kits

Supplier: HACH

Description: Accessory for test kit, Colour comparator box

J.T.Baker® Endonuclease

Supplier: Avantor

Description: J.T.Baker® Endonuclease Biotech Reagent meets the strictest cGMP standards and is designed for the degradation of both single stranded and double stranded DNA and RNA. J.T.Baker® Endonuclease Biotech Reagent is used to ensure host cell DNA impurities are removed; driving process efficiency by lowering viscosity and preventing aggregation. J.T.Baker® Endonuclease Biotech Reagent is an enzyme based upon the native endonuclease of Serratia marcescens, enabling rapid clearance of residual DNA and RNA during the production and purification of both recombinant proteins and viral vectors. Non-animal origin. Absence of proteolytic activity. The purity of materials in non-negotiable. J.T.Baker® Endonuclease Biotech Reagent acts to degrade and eliminate extraneous genetic material, ensuring the pristine quality of your final product.

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VWR®, Balance and Weight Cleaning Kit

Catalog Number: (611-2886)

Supplier: VWR Collection

Description: Cleaning kit containing a puffer brush (lens cleaning brush), cotton cloth and pair of nitrile gloves.

UOM: 1 * 1 KIT

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VWR®, Red Taq DNA polymerase

Supplier: VWR Chemicals

Description: Red Taq DNA Polymerase is a blend of Taq DNA polymerase combined with an inert red dye. The dye enables quick visual recognition of reactions to which enzyme has been added, as well as confirmation of complete mixing.

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AccuStart™ II PCR SuperMix

Supplier: Quantabio

Description: AccuStart™ II PCR SuperMix is a 2X concentrated, ready-to-use reaction cocktail for routine PCR amplification of DNA fragments up to 4 kb. It contains all components, except primers and template. AccuStart™ II PCR SuperMix simplifies reaction assembly, improves assay reproducibility, and reduces the risk of contamination. A key component is AccuStart™ II Taq DNA polymerase which contains monoclonal antibodies that bind to the polymerase and keep it inactive prior to the initial PCR denaturation step. Upon heat activation (1 minute at 94 °C), the antibodies denature irreversibly, releasing fully active, unmodified Taq DNA polymerase. This enables specific and efficient primer extension with the convenience of room temperature reaction assembly.

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VWR®, Bovine Ribonuclease A (from Pancreas)

Supplier: VWR Chemicals

Description: Ribonuclease A is an endoribonuclease that efficiently hydrolyses RNA from tissue or bacterial cell cultures.

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microRNA cDNA synthesis kit, qScript™

Catalog Number: (733-2013)

Supplier: Quantabio

Description: The qScript™ microRNA cDNA synthesis kit has been designed to convert miRNAs into cDNA starting from total RNA or RNA preparations pre-enriched for miRNAs. miRNAs are not polyadenylated in nature. With the qScript™ microRNA cDNA synthesis kit miRNAs are polyadenylated in a poly(A) polymerase reaction. qScript™ reverse transcriptase and other necessary reagents for cDNA synthesis are subsequently added to convert the poly(A) tailed microRNAs into cDNA using an oligo-dT adapter primer. The adapter primer has a unique sequence at its 5’ end which allows amplification of cDNAs in Real-Time qRT-PCR reactions.

UOM: 1 * 25 Assays

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Recombinant Proteinase K

Catalog Number: (A7932.0500)

Supplier: PanReac AppliChem

Description: Recombinant Proteinase K

UOM: 1 * 500 mg

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Pig Trypsin (from Pancreas)

Catalog Number: (A4148.0100)

Supplier: PanReac AppliChem

Description: Pig Trypsin (from Pancreas)

UOM: 1 * 100 g

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Ribonuclease A

Supplier: OMEGA BIOTEK

Description: Ribonuclease A

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VWR®, Taq DNA polymerase

Supplier: VWR Chemicals

Description: VWR® Taq DNA Polymerase is an ultra-pure, thermostable, recombinant DNA polymerase, which provides robust PCR performance in a wide range of PCR applications, without time-consuming optimisation. The enzyme is isolated from Thermus aquaticus, and has a molecular weight of approximately 94 kDa. VWR® Taq DNA Polymerase has both a 5' to 3' DNA polymerase and a double strand 5' to 3' exonuclease activity. It leaves an A overhang, which makes the enzyme ideal for TA cloning.

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Test strips, peracetic acid, Dosatest

Supplier: VWR Chemicals

Description: A ready to use kit that fits into your lab coat pocket.

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RNA isolation, E.Z.N.A.® HP total RNA kit

Supplier: OMEGA BIOTEK

Description: The E.Z.N.A.® HP Total RNA Isolation Kit provides a rapid and easy method for RNA isolation from a small amount of cultured eukaryotic cells or tissues. This kit allows single or simultaneous processing of multiple samples in less than 40 minutes. Normally, 1×10⁷ eukaryotic cells or 25 to 30 mg tissue can be used in a single experiment. There is no need for phenol/chloroform extractions, time-consuming steps such as CsCl gradient ultracentrifugation, or precipitation with isopropanol or LiCl. RNA purified using the E.Z.N.A.® HP Total RNA method is ready for applications such as RT-PCR, RT-qPCR, Northern blotting, poly A+ RNA (mRNA) purification, nuclease protection, and in vitro translation.

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