You Searched For: qPCR\/RT-qPCR+Enzymes+and+Kits

Supplier: Quantabio

Description: AccuStart™ II GelTrack PCR SuperMix is a 2X concentrated, ready-to-use reaction cocktail for routine PCR amplification of DNA fragments up to 4 kb followed by analysis on agarose gels. It contains all components, except primers and template. AccuStart™ II GelTrack PCR SuperMix simplifies reaction assembly, improves assay reproducibility, and reduces the risk of contamination. The supermix includes electrophoresis tracking dyes that migrate at approximately 4 kb and 50 bp to allow direct loading of PCR product on agarose gels following amplification. AccuStart™ II <i>Taq</i> DNA polymerase in the master mix is inactivated with monoclonal antibodies that bind the polymerase and keep it inactive prior to the initial PCR denaturation step. Upon heat activation (1 minute at 94 °C), the antibodies denature irreversibly, releasing fully active, unmodified <i>Taq</i> DNA polymerase. This enables specific and efficient primer extension with the convenience of room temperature reaction assembly.

MSDS Certificates

Supplier: OMEGA BIOTEK

Description: The E.Z.N.A.® SQ blood DNA kit provides a reliable method for the isolation of high molecular weight genomic DNA from fresh, frozen, or anti-coagulated whole blood. The method can also be used for preparation of genomic DNA from buffy coat, bone marrow or cultured cells. There is no need for phenol/chloroform extractions, and time consuming steps such as CsCl gradient ultracentrifugation are eliminated. DNA purified using the E.Z.N.A.® SQ blood DNA method is ready for downstream applications such as PCR, southern blotting and restriction enzyme digestion.

Supplier: OMEGA BIOTEK

Description: E.Z.N.A.® Cycle-Pure kits are designed for the rapid purification of single or double-stranded DNA from PCR or other enzymatic reactions. The purification procedure completely removes primers, nucleotides, enzymes, salts, and other impurities from DNA samples. E.Z.N.A.® MicroElute Cycle-Pure kits are specifically intended to purify PCR samples with a small elution volume of 10 to 15 µl. The E-Z® 96 Cycle-Pure kit procedure allows for the parallel purification of up to 96 PCR samples from multiple amplifications. The E-Z® 96 Cycle-Pure kit utilises multiwell technology for manual or fully automated high throughput purification.

MSDS

Supplier: MACRON AVANTOR BRAND

Description: Potassium dihydrogen phosphate 98.0-100.5% (dried basis, RT), crystals, GenAR® NF, Macron Fine Chemicals™

MSDS Certificates

Supplier: OMEGA BIOTEK

Description: The E.Z.N.A.® Plasmid Mini Kit I is designed to isolate up to 30 µg of high-quality plasmid DNA from 1 to 5 ml bacterial cultures in 30 minutes or less. Plasmid DNA purification is simplified with HiBind® Mini Column technology into three quick steps: Bind, wash, and elute. Purified plasmid DNA is immediately ready for a wide variety of downstream applications such as routine screening, restriction enzyme digestion, and manual/automated fluorescent DNA sequencing. E.Z.N.A.® Plasmid Mini Kit I is available in either V-spin or Q-spin column format. V-spin column formats have an attached lid while Q-spin columns do not. Both versions can be used for centrifugation or vacuum protocols.

MSDS

Supplier: OMEGA BIOTEK

Description: E.Z.N.A.® Plasmid Midi Kit offers a novel and reliable method of isolating up to 200 µg of plasmid DNA in less than 60 minutes. This kit combines HiBind® membrane technology with the time-tested consistency of alkaline SDS lysis of bacterial cells to deliver high-quality plasmid DNA. The DNase/RNase-free HiBind® DNA midi columns operate by eliminating time consuming phenol-chloroform extractions and alcohol precipitations. Plasmid purification using the E.Z.N.A.® Plasmid Midi Kit follows a simple bind-wash-elute procedure thereby making it possible for multiple samples to be processed. Although yields vary according to plasmid copy number, <i>E.coli </i>strain, and conditions of growth, 50 ml of an overnight culture in LB medium will typically produce 200 µg of high-copy number plasmid DNA. When working with low-copy number plasmids the starting culture volume can be increased up to 100 ml. High-quality DNA is ready for immediate use in routine molecular biology applications, such as automated fluorescent sequencing, restriction enzyme digestion, and transfection screening.

MSDS

Supplier: OMEGA BIOTEK

Description: By adapting HiBind® technology with 96-well plates, the E-Z 96® Tissue DNA kit provides a high throughput method to purify genomic DNA from whole blood, buccal swabs, mouse tail, rat tail, tissues and animal cells and tissues in a 96-well plate format. Purified DNA is suitable for most downstream applications such as PCR, restriction enzyme digestion, and hybridisation techniques.

MSDS

Supplier: VWR Collection

Description: Stainless steel baths with user friendly MX temperature controller with three control buttons and on-screen prompts.

Supplier: VWR Collection

Description: Transparent polycarbonate baths keep samples visible, units feature user friendly MX temperature controller with three control buttons and on-screen prompts.

Catalog Number: (733-2903)

Supplier: Quantabio

Description: Faster, better, tougher next generation cDNA SuperMix for cDNA synthesis.

UOM: 1 * 1 KIT

Sale

Catalog Number: (462-0214)

Supplier: VWR Collection

Description: Stainless steel bath with user friendly MX temperature controller, has three control buttons and on-screen prompts. Specifically designed for production of microbiological culture media. The controller bridge, made from chemical resistant DuraTop™, is easily removed to allow cleaning of the tank. The bath has an opening with lid for optional cooling coil, which is ideal for set points near ambient temperature.

UOM: 1 * 1 items

Sale

Catalog Number: (462-0205)

Supplier: VWR Collection

Description: The MX immersion thermostat is user friendly with three control buttons and on-screen prompts. The convenient slide control enables the flow rate to be easily adjusted, and it can clamp securely to straight or curved tank walls.

UOM: 1 * 1 items

Sale

Supplier: VWR Collection

Description: Multi-purpose, high wattage, constant temperature, VWR® analogue block heaters are economical, versatile and compact.

Supplier: VWR Collection

Description: Small, low cost recirculator that handles applications such as thawing plasma and tempering photographic solutions. The sealed reservoir can control closed loop systems only.

Supplier: OMEGA BIOTEK

Description: The E.Z.N.A.® blood DNA kit provides rapid total DNA isolation from up to 250 µl of fresh or frozen anticoagulated whole blood. The E.Z.N.A.® blood DNA kit can also be used for the preparation of genomic DNA from buffy coat, serum, plasma, bone marrow, lymphocytes, platelets, and body fluids. This kit allows for simultaneous processing of single or multiple samples in less than 30 minutes. Phenol/chloroform extractions, and time-consuming steps such as precipitation with isopropanol or ethanol have been eliminated. DNA purified with the E.Z.N.A.® blood DNA method is ready for applications such as PCR, southern blotting, or restriction enzyme digestion.

MSDS

Supplier: VWR Collection

Description: Stainless steel circulating baths with choice of Advanced Programmable or Advanced Digital temperature controller. Both are easy to navigate with large, intuitive displays and multiple communication options including USB-A and B, RS232/485, Ethernet and external temperature probe. The reservoir drain can be accessed by removing the front panel. All models feature user-adjustable high temperature safety cut-off points as well as over-temperature protection. The DuraTop™ surface is cool to the touch even when operating at high temperatures.