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Catalog Number: (L0042-500)
Supplier: BIOWEST
Description: Basal Medium Eagle is principally used for monolayer diploid or primary cell cultures. It is the simplest of the basal media with all essential components for growth. BME ideally supports cell lines such as HeLa, L-cells and primary mammalian fibroblasts. BME has also successfully been used for the growth of human transformed cell lines (particularly WI-38 and MRC-5).
UOM: 1 * 500 mL

Supplier: BIOWEST
Description: Studies to determine the nutritional requirements of many cells have been in progress since Eagle's first reports. The major essential nutrients were identified, and work became focussed on the media requirements of individual cell types. Many media designed for these purposes are now available. Among the first of these media, developed initially to study hormonal requirements of cells in culture, was a mixture of DMEM and Ham's F12 medium, known as DMEM-Ham's F12.
Catalog Number: (L0207-500)
Supplier: BIOWEST
Description: Schneider’s Insect Medium was developed to support the growth of excised imaginal discs from the fruit fly, <i>Drosophila melanogaster</i>. When supplemented with 5 to 20% heat-inactivated foetal bovine serum Schneider's mediuam has been found to support the rapid growth of both primary and established cultures of cells derived from Drosophila spp. and several other dipterans.
UOM: 1 * 500 mL

Catalog Number: (J869-500G)
Supplier: VWR Chemicals
Description: Terrific broth is an enriched medium, ideal for culturing cells for nucleic acid purification and recombinant protein expression.
UOM: 1 * 500 g

Supplier: BIOWEST
Description: Glasgow MEM (GMEM) was originally formulated for growing BHK 21 cells. The original formulation is an Eagle's Basal Medium modified to contain two times the normal concentration of amino acids and vitamins. It is intended for use with adherent cell lines.

Catalog Number: (M307-1ML)
Supplier: VWR Chemicals
Description: Contains a mix of protease inhibitors: AEBSF, E-64, Bestatin, Pepstatin, Leupeptin and 1, 10-Phenanthroline. Each vial can be reconstituted in 1 ml dimethyl sulfoxide to form a 100X solution.
UOM: 1 * 1 mL


Supplier: BIOWEST
Description: In 1976, Guilbert and Iscove demonstrated that precursor cells of erythrocytes and macrophages could be cultured in a reduced-serum medium supplemented with albumin, transferrin, lecithin, and selenium. Iscove's medium is a modification of Dulbecco's Modified Eagle's Medium (DME) containing selenium, additional amino acids and vitamins, sodium pyruvate, HEPES buffer, and potassium nitrate instead of ferric nitrate. Further studies have demonstrated that Iscove's medium will support murine B lymphocytes, haemopoietic tissue from bone marrow, B-cells stimulated with lipopolysaccharide, T-lymphocytes, and a variety of hybrid cells.
Supplier: BIOWEST
Description: L-glutamine is an essential amino acid required by virtually all mammalian and insect cells grown in culture. It is a crucial component of many cell culture media and serves as a major energy source for cells in culture. L-glutamine is very stable as a dry powder and as a frozen solution. However, in liquid media or stock solutions, L-glutamine can degrade relatively rapidly. L-glutamine is also more labile in cell culture solution than other amino acids.
Supplier: VWR Collection
Description: Square PETG bottles are ideal for storage of media, laboratory reagents and biological buffers up to −70 °C. Eliminate washing, depyrogenation and autoclaving steps with sterile and non-pyrogenic.
Supplier: VWR Collection
Description: PC, autoclavable, narrow mouth bottles with standard PP caps.

Supplier: VWR Collection
Description: PS, PC or PETG, with PP screw cap, sterile.

Catalog Number: (X0556-100)
Supplier: BIOWEST
Description: Concentrated solutions of amino acids, vitamins or non essential amino acids can be added to a basal medium to enrich it and the resulting medium used as a classical MEM or BME. The final concentration of the MEM or BME must be 1X.
UOM: 1 * 100 mL


Catalog Number: (P5030-500GR)
Supplier: BIOWEST
Description: D-glucose monohydrate (Dextrose) is a common natural sugar involved in processes such as energy production, glycosylation, and formation of glycans that provide structure to cells. It is involved in a detrimental process in cells called glycation. It is used as a supplement for cell culture and in numerous cellular processes.
UOM: 1 * 500 g

Supplier: BIOWEST
Description: Cell culture water is high-quality water suitable for use as a solvent in the preparation of cell culture media and laboratory reagents.
Supplier: BIOWEST
Description: Trypsin is a porcine pancreas-derived enzyme that is commonly used for the dissociation and disaggregation of anchorage-dependent mammalian cells and tissues. The concentration of trypsin necessary to dislodge the cells from their substrate depends on the sensitivity of the cells.
Catalog Number: (A3732.0500)
Supplier: PanReac AppliChem
Description: Water-soluble part of autolysed yeast. Rich of ammonia and vitamins, especially of water-soluble vitamin B-complexes; suitable for addition to media.
UOM: 1 * 500 g

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Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us at 1-800-932-5000.
This product is marked as restricted and can only be purchased by approved Shipping Accounts. If you need further assistance, email VWR Regulatory Department at eurega_services@eu.vwr.com
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This product is no longer available. Alternatives may be available by searching with the VWR Catalog Number listed above. If you need further assistance, please call VWR Customer Service at 1-800-932-5000.
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