You Searched For: Transformation+Reagents

Supplier: VWR Chemicals

Description: Reagent for the preparation of competent cells for transformation.

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Supplier: VWR Chemicals

Description: A sterile reagent for the preparation of competent cells for transformation.

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Supplier: MIRUS

Description: Ingenio® Electroporation solution is compatible with many conventional electroporation instruments, including Lonza Amaxa® Nucleofector®, Bio-Rad® Gene Pulser and Harvard-BTX® electroporators. It can be used for electroporation with both exponential decay as well as square wave forms.

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Supplier: VWR Chemicals

Description: Super optimal broth (SOB) broth is used for the preparation of nutritionally-rich growth medium and transformation of competent <i>E. coli</i>.

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Catalog Number: (N400-25PK)

Supplier: VWR Chemicals

Description: Bacterial freezing media is a sterile, ready to use cryopreservation media for long term storage of bacterial cultures. Ideal for the preservation of transformed bacteria as well as non fastidious bacteria.

UOM: 1 * 25 items

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Catalog Number: (N549-6X5ML)

Supplier: VWR Chemicals

Description: SOC medium is used in the final step of bacterial cell transformation to obtain maximum transformation efficiency of <i>E. coli</i> SOC.

UOM: 1 * 1 KIT

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Supplier: BIOWEST

Description: The long term cultivation of cells requires the addition of serum. When properly supplemented, Medium 199 has broad species applicability, particularly for cultivation of non-transformed cells. It is widely used in virology, vaccine production and<i> in vitro</i> cultivation of primary explants of mouse pancreatic epithelial and rat lens tissues.

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Supplier: BIOWEST

Description: McCoy’s media were originally formulated for growth and support of lymphocytes. This final modification produced a medium identical to RPMI 1629. McCoy's 5A media supports the indefinite proliferation of Walker 256 carcinoma cells. In addition, it is excellent for the propagation of leukocytes, biopsy tissues, a broad range of human and rat normal or transformed cell types, the most current primary and continuous cell lines.

Catalog Number: (L0042-500)

Supplier: BIOWEST

Description: Basal Medium Eagle is principally used for monolayer diploid or primary cell cultures. It is the simplest of the basal media with all essential components for growth. BME ideally supports cell lines such as HeLa, L-cells and primary mammalian fibroblasts. BME has also successfully been used for the growth of human transformed cell lines (particularly WI-38 and MRC-5).

UOM: 1 * 500 mL

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Supplier: BIOWEST

Description: Minimum Essential Medium (MEM) with Earle's Balanced Salts is a modification of Eagle's earlier medium, Basal Medium Eagle (BME), containing higher concentrations of the essential nutrients. These media have demonstrated the ability to support a variety of normal and transformed cells in culture and contain Earle's Balanced Salts, which make them suitable for use in atmospheres charged with CO₂ gas.

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Supplier: VWR Chemicals

Description: Agarose high resolution (HR) has melting, max. 90 °C, and gelling temperatures (32,5 to 38 °C), but differs from traditional low melting point molecular biology grade agaroses in the gel separation range. Using this agarose in electrophoresis allows fine and consistent resolution of nucleic acids below 1000 base pairs, which differ by only a few base pairs. The properties of Agarose HR allow consistent gel separations (analytical and preparative) and performance of <i>in-vitro</i> translation and transcription mapping, as well as <i>in-vivo</i> ligation and transformation. For analytical gel separation of DNA/RNA below 1000 base pairs, where a higher gel strength for ease of manipulation is required, we recommend the use of Agarose, high resolution, Electran (43655).

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Supplier: VWR Collection

Description: Shaking bath, Stainless steel base tray (converts bath to unstirred bath), for VLSB12/VKB12

Supplier: Corning

Description: Medium 199 is often used in vaccine production, virology, and for the culture of various non-transformed cells

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Catalog Number: (VWRU89047-208)

Supplier: VWR Collection

Description: High quality durable polycarbonate has been tested to optimise transformation efficiencies. These cuvettes are built to provide exact tolerances ensuring reproducible results.

UOM: 1 * 50 items

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Catalog Number: (231-0606)

Supplier: BÜRKLE

Description: PS. Double-scoop spatulas with rounded ends, content 1,0 and 0,25 ml. Can be used to sample, transfer or process small amounts of chemicals, powders, granulates, pastes, creams or liquids.

UOM: 1 * 100 items

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Supplier: VWR Collection

Description: This PCR storage rack offers a sleek and modern design that includes a unique lid that the rack slides out from, and then rotates 180°, to become a convenient stand that the rack then sits on. Ergonomic, textured finger grips provide a secure hold, reducing the chance of dropping the PCR rack, making handling and transportation easy, especially when using gloves.