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VWR® Transfer Buffer Concentrate, CAPS 10X

Catalog Number: (K872-500ML)

Supplier: VWR Chemicals

Description: For transfer of protein to membranes for sequencing or blotting.

UOM: 1 * 500 mL

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VWR®, Rapid Transfer buffer 10X

Catalog Number: (N789-4L)

Supplier: VWR Chemicals

Description: Rapid transfer buffer is a simple, single component system for quick and efficient transfer of proteins from SDS-PAGE gels to membranes for Western blotting applications. Transfer is completed in 10 or 20 minutes using a standard semi-dry or wet transfer apparatus, respectively.

UOM: 1 * 4 L

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VWR® Proteomics Grade, Tris-glycine buffer

Catalog Number: (M115-40L)

Supplier: VWR Chemicals

Description: A pre-blended Tris-glycine powder for preparation of Laemmli SDS-PAGE running buffer. The 40 litre pack size prepares 40 litres of 1X Tris-glycine solution (0,025 M Tris base and 0,192 M glycine). Each Ready-Pack™ prepares 1 litre of a 10X concentrate.

UOM: 1 * 40 L

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VWR® PerfectBlue, Tank Blotters

Supplier: VWR Collection

Description: PerfectBlue S and M tank electroblotters are designed to rapidly transfer nucleic acid or protein fragments from up to four polyacrylamide gels at a time to nitrocellulose, nylon or PVDF membranes. The colour coded cassettes allow for easy assembly of transfer sandwich and error-free transfer. The large buffer capacity and integral cooling allow extended transfer times and high voltage/high current operation for the transfer of high molecular weight proteins.

VWR® kuroGEL, Semi-dry blotter

Catalog Number: (700-0416)

Supplier: VWR Collection

Description: The kuroGEL semi-dry blotter provides great flexibility in a laboratory since it can be used for all types of blotting: Western, Southern and Northern. Blotting is fast and easy, and can typically be completed in 15 to 30 min. Set-up procedures are easy and economical with little buffer needed. The screw down lid adjusts to varying gel thicknesses and sizes, while the platinum coated electrodes provide uniform pressure ensuring even transfers.

UOM: 1 * 1 items

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VWR®, Blotting Pads, Grade 703

Supplier: VWR Collection

Description: Ideal for use as wicks in protein and nucleic acid blotting. Provides a uniform flow of buffer through the gel to the transfer membrane in a blotting sandwich. Also suitable for use in removing gels from glass supports.

VWR®, Blotting Pads, Grade 707

Supplier: VWR Collection

Description: This super thick paper is used to complete the blotting sandwich. Smooth, uniform texture ensures continuous contact of buffer from the chamber through the gel and transfer membrane.

RNA isolation, total RNA kit, E-Z 96®

Supplier: OMEGA BIOTEK

Description: The E-Z 96® Total RNA Kit is designed for isolation of total cellular RNA from up to 5×10⁶ cultured cells or soft tissues. This kit can process single or multiple samples in less than 60 minutes. Utilising HiBind® silica plate technology, the need for phenol/chloroform extractions, CsCl gradient ultracentrifugation, and precipitation with isopropanol or LiCl are eliminated. Samples are lysed in a denaturing lysis buffer which inactivates RNases. Binding conditions are adjusted and the lysate is transferred to a 96-well HiBind® RNA Plate where the RNA is purified via three wash steps. High quality RNA is eluted in RNase-free water. RNA purified using the E-Z 96® Total RNA method is ready for applications such as RT-PCR, qPCR, differential display, microarrays, and other downstream applications.

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Mag-Bind® PX blood RNA 96 kit

Supplier: OMEGA BIOTEK

Description: The Mag-Bind® PX blood RNA kit allows for the isolation of total RNA from 2,5 ml of whole blood that has been stored in PAXgene® Blood RNA tubes. Stabilised blood samples are first spun down and the nucleic acid pellets are washed and recollected. After washing, the nucleic acid pellet is dissolved into a specially formulated resuspension buffer. At this point the samples can be transferred to 96-well plates for processing in robotic liquid handlers and magnetic processors. High quality intact RNA can be isolated in less than 90 minutes.