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Supplier: OMEGA BIOTEK

Description: The E.Z.N.A.® Plasmid Maxi Kit is the maxi scale system to deliver high-quality plasmid DNA using the spin column format, without costly accessories. The HiBind® DNA Maxi column facilitates the binding, washing, and elution steps, thus enabling multiple samples to be simultaneously processed. Although yields vary according to plasmid copy number, <i>E.coli</i> strain, and conditions of growth, 50 to 200 ml of overnight culture in LB medium typically produces 0,5 to 1 mg of high copy number plasmid DNA. Up to 500 ml of culture may be processed when working with low copy number plasmid. The product is suitable for automated fluorescent DNA sequencing (typical reads exceed 800 bp), restriction enzyme digestion, transfection of mammalian cells, transcription<i> invitro</i>, and other applications.

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Supplier: OMEGA BIOTEK

Description: The E.Z.N.A.® Plasmid Mini Kit I is designed to isolate up to 30 µg of high-quality plasmid DNA from 1 to 5 ml bacterial cultures in 30 minutes or less. Plasmid DNA purification is simplified with HiBind® Mini Column technology into three quick steps: Bind, wash, and elute. Purified plasmid DNA is immediately ready for a wide variety of downstream applications such as routine screening, restriction enzyme digestion, and manual/automated fluorescent DNA sequencing. E.Z.N.A.® Plasmid Mini Kit I is available in either V-spin or Q-spin column format. V-spin column formats have an attached lid while Q-spin columns do not. Both versions can be used for centrifugation or vacuum protocols.

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Supplier: OMEGA BIOTEK

Description: The E.Z.N.A.® Blood RNA Kit is designed for the isolation of total intracellular RNA from up to 1 ml of fresh, or frozen whole blood treated with any common anticoagulant such as heparin, EDTA or acid-citrate-dextrose. The procedure completely removes contaminants and enzyme inhibitors making total RNA isolation fast, convenient and reliable. Red blood cells are selectively lysed and white cells are collected by centrifugation. After lysis of white blood cells under denaturing conditions that inactivate RNases, the lysate is homogenised with a homogeniser spin column. The sample is then applied to a HiBind® spin column. Cellular debris and other contaminants such as haemoglobin are effectively washed away and high-quality RNA is finally eluted in DEPC treated water.

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Catalog Number: (D2492-03)

Supplier: OMEGA BIOTEK

Description: The E.Z.N.A.® Blood DNA Maxi Kit is specially designed for large scale isolation of genomic DNA. The kit provides a rapid purification of genomic DNA from up to 10 ml whole blood samples. Sample sources include fresh and frozen whole blood treated with common anticoagulants such as citrate, EDTA and heparin. In addition plasma, serum, buffy coat, bone marrow, lymphocytes, platelets, and body fluid samples can also be used. Phenol/chloroform extractions, and time-consuming steps such as precipitation with isopropanol have been eliminated. DNA purified using the E.Z.N.A.® Blood DNA Maxi method is free of contaminants and enzyme inhibitors making it suitable for most downstream applications such as PCR, Southern blotting and restriction enzyme digestion of high-quality total DNA.

UOM: 1 * 50 Tests

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Supplier: OMEGA BIOTEK

Description: Plasmid isolated with traditional purification procedures normally contain high levels of endotoxins (also known as lipopolysaccharides or LPS) that can significantly interfere with transfection experiments downstream. The E.Z.N.A.® Endo-Free plasmid mini kit II integrates an efficient endotoxin removal step into the plasmid purification procedure to produce high-quality transfection grade (<0,1 EU/µg) plasmid for efficient transfection. The bacterial cells are lysed using the alkaline-SDS lysis method. The cleared cell lysate is then treated with ETR reagent to efficiently remove the endotoxins. After adjusting the binding condition, the cell lysate is applied into the HiBind® DNA column and purified DNA is eluted from the column membrane.

Supplier: OMEGA BIOTEK

Description: The E.Z.N.A.® bacterial DNA kit allows the rapid and reliable isolation of high quality total cellular DNA from a wide variety of bacterial species. This kit uses optimised lysis condition and up to 1×10⁹ bacterial cells can be processed for each column. There are no organic extractions, thus reducing plastic waste and hands on time to allow multiple samples to be processed in parallel. Bacterial cells are grown to log-phase and harvested. The cell wall is removed by lysozyme digestion and bead beating, followed by protease digestion. Following lysis, binding conditions are adjusted and the sample is applied to a HiBind DNA spin-column. Two rapid wash steps remove trace salts and protein contaminants, and DNA is finally eluted in water or elution buffer. Purified DNA can be directly used in downstream applications without the need for further purification.

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Catalog Number: (113-8151)

Supplier: NITRITEX

Description: These disposable aprons are made of CleanTough™ fabric, a spunbonded non woven PP fabric laminated with a PE film.

UOM: 1 * 50 items

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Catalog Number: (111-2383)

Supplier: VWR Collection

Description: Cords and pouches for safety eyewear spectacles.

UOM: 1 * 10 items

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Catalog Number: (AC-1370-00)

Supplier: OMEGA BIOTEK

Description: Nucleic Acid Purification Kits and Reagents, Collection tube, 2 ml, capless, graduated

UOM: 1 * 5.000 items

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Supplier: OMEGA BIOTEK

Description: Isolate plasmid DNA from up to 5 ml culture in 9 minutes utilizing an innovative lysate clearance column.

Supplier: OMEGA BIOTEK

Description: E.Z.N.A.® Plasmid Midi Kit offers a novel and reliable method of isolating up to 200 µg of plasmid DNA in less than 60 minutes. This kit combines HiBind® membrane technology with the time-tested consistency of alkaline SDS lysis of bacterial cells to deliver high-quality plasmid DNA. The DNase/RNase-free HiBind® DNA midi columns operate by eliminating time consuming phenol-chloroform extractions and alcohol precipitations. Plasmid purification using the E.Z.N.A.® Plasmid Midi Kit follows a simple bind-wash-elute procedure thereby making it possible for multiple samples to be processed. Although yields vary according to plasmid copy number, <i>E.coli </i>strain, and conditions of growth, 50 ml of an overnight culture in LB medium will typically produce 200 µg of high-copy number plasmid DNA. When working with low-copy number plasmids the starting culture volume can be increased up to 100 ml. High-quality DNA is ready for immediate use in routine molecular biology applications, such as automated fluorescent sequencing, restriction enzyme digestion, and transfection screening.

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Supplier: OMEGA BIOTEK

Description: The Mag-Bind® oligonucleotide purification kit is designed for the purification of small size DNA fragments (larger than 10 bp) with a 60 to 70% recovery rate. By using the highly efficient binding ability of Mag-Bind® technology, oligonucleotides are selectively bound to the surface of Mag-Bind® particles. Salts and other impurities are washed away with two quick wash steps. Purified oligouncleotide are eluted with deionised water or elution buffer and ready for downstream applications.

Supplier: OMEGA BIOTEK

Description: Mag-Bind® SeqDTR™ is designed for the effective and reliable removal of unincorporated terminators from sequencing reaction. Sequencing products are first mixed with the Mag-Bind® SeqDTR™. DNA then selectively binds to the Mag-Bind® SeqDTR™ particles. With two rapid wash steps, trace contaminants such as nucleotides, primers and small, non targeted amplification products are removed.

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Catalog Number: (VWRMNPSL403)

Supplier: VWR USA

Description: A buffered alkaline rinse with a mild pH (8,0).

UOM: 1 * 1 items

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Supplier: VWR Collection

Description: Frame: Stainless steel; woven wire cloth: Stainless steel, ISO 3310, ASTM E11, BS 410, AFNOR.

Catalog Number: (M1386-01)

Supplier: OMEGA BIOTEK

Description: Mag-Bind® RXNPure Plus Kit allows rapid and reliable isolation DNA from PCR and enzymatic reactions with high recovery rates. The system combines proprietary chemistries with the reversible nucleic acid-binding properties of magnetic beads that selectively bind PCR amplicons 100 bp and larger, and eliminate excess nucleotides, primers and small, non targeted amplification products, such as primer dimers.

UOM: 1 * 50 mL

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