You Searched For: RT-PCR+and+cDNA+Reagents

Supplier: VWR Chemicals

Description: VWR® TEMPase Hot Start DNA Polymerases are highly stable polymerases, featuring higher specificity, superior sensitivity and greater yields compared to standard DNA polymerases. These features make them well suited for the detection of low abundance targets. Other uses include screening, amplification of GC-rich sequences, multiplex PCR, direct PCR and qPCR. A glycerol-free TEMPase Hot Start DNA Polymerase is also available for automation and freeze drying.

Catalog Number: (E542-40UM)

Supplier: VWR Chemicals

Description: Deoxyribonucleotides are the foundation of DNA and it takes thousands of these nucleotides to make up DNA.

UOM: 1 * 40 µmol

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Supplier: Quantabio

Description: Superior speed and inhibitor tolerance.

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Catalog Number: (VWRA06105100)

Supplier: VWR Collection

Description: VWR® Delivered High quality Reagents & biochemicals, combined with innovative products that gives optimum results , save time & simplify protocols

UOM: 1 * 1 KU

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Catalog Number: (K461-KIT)

Supplier: VWR Chemicals

Description: Set contains one vial of each dNTP (dATP, dTTP, dCTP, dGTP), 40 μmol of 100 mM solution, pH 7,0.

UOM: 1 * 1 KIT

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Supplier: Quantabio

Description: AccuStart™ Genotyping ToughMix® is a 2X concentrated ready to use reaction cocktail for PCR amplification of DNA templates that overcomes inhibitors often present in crude samples extracted from environmental specimens, plant tissues, or animal tissues. It is a versatile and robust PCR reagent that provides highly specific and efficient amplification for genotyping sequence variants including allelic discrimination of single nucleotide polymorphisms (SNPs) using a variety of fluorogenic probe chemistries, including TaqMan® hydrolysis probes.

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Catalog Number: (E636-40UMOLE)

Supplier: VWR Chemicals

Description: Ready to use dNTP mix. One tube of 10 μmol of each dNTP in a 25 mM solution, pH 7,0. Tube volume 400 μl.

UOM: 1 * 40 µmol

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Supplier: Quantabio

Description: AccuStart™ II <i>Taq</i> DNA Polymerase is a high purity, recombinant <i>Taq</i> DNA polymerase preparation with high avidity monoclonal antibodies that bind the polymerase and keep it inactive prior to the initial PCR denaturation step. Upon heat activation (1 minute at 94 °C), the antibodies denature irreversibly, releasing fully active, unmodified <i>Taq</i> DNA polymerase. This enables specific and efficient primer extension with the convenience of room temperature reaction assembly. Non-specific extension of primers at low temperatures is a common cause of artifacts and poor sensitivity in PCR. The AccuStart™ II automatic hot-start enables specific and efficient primer extension in the PCR process with the added convenience of room temperature reaction assembly. The included 10X PCR Buffer II is a new optimised buffer that provides higher product yield, improved specificity, and enhanced multiplexing capability.

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Supplier: Quantabio

Description: AccuStart™ II PCR SuperMix is a 2X concentrated, ready-to-use reaction cocktail for routine PCR amplification of DNA fragments up to 4 kb. It contains all components, except primers and template. AccuStart™ II PCR SuperMix simplifies reaction assembly, improves assay reproducibility, and reduces the risk of contamination. A key component is AccuStart™ II Taq DNA polymerase which contains monoclonal antibodies that bind to the polymerase and keep it inactive prior to the initial PCR denaturation step. Upon heat activation (1 minute at 94 °C), the antibodies denature irreversibly, releasing fully active, unmodified Taq DNA polymerase. This enables specific and efficient primer extension with the convenience of room temperature reaction assembly.

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Supplier: Quantabio

Description: AccuStart™ II GelTrack PCR SuperMix is a 2X concentrated, ready-to-use reaction cocktail for routine PCR amplification of DNA fragments up to 4 kb followed by analysis on agarose gels. It contains all components, except primers and template. AccuStart™ II GelTrack PCR SuperMix simplifies reaction assembly, improves assay reproducibility, and reduces the risk of contamination. The supermix includes electrophoresis tracking dyes that migrate at approximately 4 kb and 50 bp to allow direct loading of PCR product on agarose gels following amplification. AccuStart™ II <i>Taq</i> DNA polymerase in the master mix is inactivated with monoclonal antibodies that bind the polymerase and keep it inactive prior to the initial PCR denaturation step. Upon heat activation (1 minute at 94 °C), the antibodies denature irreversibly, releasing fully active, unmodified <i>Taq</i> DNA polymerase. This enables specific and efficient primer extension with the convenience of room temperature reaction assembly.

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Catalog Number: (733-2265)

Supplier: Quantabio

Description: PerfeCTa® PreAmp SuperMix is a 5X concentrated, ready to use reaction cocktail for unbiased, selected enrichment of target sequences from limited amounts of starting material for downstream gene expression profiling or targeted resequencing. It contains all components, except primers and templates. The 5X concentrated SuperMix allows addition of higher template volumes when working with low concentration samples, and/or reduced reaction volumes. Inclusion of an inert light blue tracer dye helps visualise small reaction volumes and ensure accurate pipetting.

UOM: 1 * 40 Assays

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Supplier: VWR Chemicals

Description: Ready to use molecular biology grade dNTP mixes and dNTP sets. The dNTP mix is designed to save hands-on time for researchers, and reduce the possibility of contamination by reducing pipetting. dNTP solutions are also available in sets of four individual dNTPs, each 100 mm. Both are convenient for use in DNA polymerisation reactions, DNA labelling and sequencing processes.

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Supplier: VWR Chemicals

Description: Ribozol™ RNA Extraction Reagent is a single phase phenol solution optimized for the isolation of total RNA from a variety of cell and tissue types.

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Catalog Number: (VWRA06123410)

Supplier: VWR Collection

Description: VWR® Delivered High quality Reagents & biochemicals, combined with innovative products that gives optimum results , save time & simplify protocols

UOM: 1 * 200 µl

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Catalog Number: (VWRA06125100)

Supplier: VWR Collection

Description: VWR® Delivered High quality Reagents & biochemicals, combined with innovative products that gives optimum results , save time & simplify protocols

UOM: 1 * 1 mL

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Catalog Number: (VWRA06145100)

Supplier: VWR Collection

Description: VWR® Delivered High quality Reagents & biochemicals, combined with innovative products that gives optimum results , save time & simplify protocols

UOM: 1 * 1 mL

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