You Searched For: RT-PCR+and+cDNA+Reagents

Catalog Number: (1B1326-50RXN)

Supplier: VWR Chemicals

Description: Ready One-Step RT-PCR Kit offers a single step procedure for performing RT-PCR reactions. Subsequent loading and visualisation of RT-PCR products are streamlined since the loading buffer and visualisation dye are included. The user supplies the primers and the DNase-treated template RNA.

UOM: 1 * 1 KIT

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Supplier: Quantabio

Description: The qScript™ XLT One-Step RT-PCR Kit is a convenient and highly sensitive system for amplification of RNA templates up to 2 kb. cDNA synthesis and PCR amplification are carried out in the same tube without opening between procedures. This system has been optimised to deliver maximum endpoint RT-PCR efficiency, sensitivity, and specificity.

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Supplier: Quantabio

Description: qScript™ XLT cDNA SuperMix is a next generation tool for first-strand cDNA synthesis, providing a highly sensitive and easy to use solution for two-step RT-PCR and RT-qPCR. qScript XLT is an engineered M-MLV reverse transcriptase with reduced RNase H activity and improved activity and stability at higher temperatures. Combined with a precise mixture of reaction components, this SuperMix enables superior results over a wide dynamic range of input RNA, with up to 8-fold higher sensitivity than cDNA synthesis kits utilising an RNase H(+) reverse transcriptase (RT).

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Catalog Number: (733-2013)

Supplier: Quantabio

Description: The qScript™ microRNA cDNA synthesis kit has been designed to convert miRNAs into cDNA starting from total RNA or RNA preparations pre-enriched for miRNAs. miRNAs are not polyadenylated in nature. With the qScript™ microRNA cDNA synthesis kit miRNAs are polyadenylated in a poly(A) polymerase reaction. qScript™ reverse transcriptase and other necessary reagents for cDNA synthesis are subsequently added to convert the poly(A) tailed microRNAs into cDNA using an oligo-dT adapter primer. The adapter primer has a unique sequence at its 5’ end which allows amplification of cDNAs in Real-Time qRT-PCR reactions.

UOM: 1 * 25 Assays

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Supplier: Quantabio

Description: qScript™ cDNA synthesis kit contains qScript™ Reverse Transcriptase, a mixture of a MMLV reverse transcriptase derivative and ribonuclease inhibitor protein, optimised for sensitive and reliable cDNA synthesis over a broad dynamic range of input RNA. cDNA synthesis reagents are provided in a variety of SuperMix and kit formats to suit specific applications.

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Catalog Number: (733-2903)

Supplier: Quantabio

Description: Faster, better, tougher next generation cDNA SuperMix for cDNA synthesis.

UOM: 1 * 1 KIT

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Supplier: Quantabio

Description: Synthesis of cDNA and PCR amplification are carried out in the same tube without opening between procedures. These kits are optimised to deliver maximum RT-PCR efficiency and specificity, enabling unbiased co-amplification of low copy transcripts in the presence of higher copy reference genes. As a result the qScript™ One-Step qRT-PCR Kits offer improved sensitivity over other commercially available qPCR reagents. The qScript™ One-Step Master Mix maximises the activities of both reverse transcriptase and PCR enzymes, while minimising primer-dimer formation. High specificity is assured with AccuStart™ <i>Taq</i> DNA Polymerase, which is bound by monoclonal antibodies for superior hot-start. Upon heating to 95 °C, the antibodies denature irreversibly releasing fully active and unmodified <i>Taq</i> DNA polymerase. qScript™ One-Step qRT-PCR Kits are available for all Real-Time PCR instrument platforms, including those requiring normalisation with ROX reference dye.

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Supplier: Quantabio

Description: The AccuStart™ II Mouse Genotyping kit is designed for fast and easy preparation of PCR-ready DNA extracts and endpoint PCR analysis from tissues such as tail snips and ear punches commonly used for genotyping knockout and transgenic animals. The kit combines Extracta™ DNA Prep for PCR - Tissue with AccuStart™ II GelTrack™ PCR SuperMix in a single kit.

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Supplier: Quantabio

Description: qScript™ XLT One-Step RT-qPCR ToughMix® is a ready to use, highly sensitive master mix for reverse transcription quantitative PCR (RT-qPCR) of RNA templates.

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Supplier: VWR Collection

Description: VWR® PCR Classic plasticwares are DNAse-, RNAse- and endotoxin-free for hassle free application outcome in PCR labs.

Catalog Number: (1B1409-100RXN)

Supplier: VWR Chemicals

Description: Hot Start PCR-To-Gel Taq Master Mix, 2X is a ready to use reaction cocktail that contains all required components, except primers and template DNA, for routine PCR amplification of DNA fragments up to 4 kb.

UOM: 1 * 1 KIT

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Supplier: Quantabio

Description: AccuStart™ II PCR SuperMix is a 2X concentrated, ready-to-use reaction cocktail for routine PCR amplification of DNA fragments up to 4 kb. It contains all components, except primers and template. AccuStart™ II PCR SuperMix simplifies reaction assembly, improves assay reproducibility, and reduces the risk of contamination. A key component is AccuStart™ II Taq DNA polymerase which contains monoclonal antibodies that bind to the polymerase and keep it inactive prior to the initial PCR denaturation step. Upon heat activation (1 minute at 94 °C), the antibodies denature irreversibly, releasing fully active, unmodified Taq DNA polymerase. This enables specific and efficient primer extension with the convenience of room temperature reaction assembly.

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Catalog Number: (VWRA06105500)

Supplier: VWR Collection

Description: VWR® Delivered High quality Reagents & biochemicals, combined with innovative products that gives optimum results , save time & simplify protocols

UOM: 1 * 200 Tests

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Catalog Number: (N806-2X1.25ML)

Supplier: VWR Chemicals

Description: Ready PCR Mix, 2X offers a single-step procedure for performing PCR reactions followed by analysis on agarose gels without the addition of loading buffer. All components for assembly and performance of PCR reactions as well as loading and visualisation of PCR products on agarose gels are included. The user supplies primer and template DNA.

UOM: 1 * 1 KIT

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Supplier: Avantor

Description: PCR 8-tube strips and 8-cap strips are made of high-quality PP resin. Clear caps are compatible with qPCR application.

Supplier: Quantabio

Description: qScript™ One-Step SYBR® Green qRT-PCR Kits reliably deliver maximum qRT-PCR efficiency, sensitivity and specificity in with a simplified protocol. These kits offer reliable and unbiased amplification of all RNA targets regardless of abundance. The proprietary supermix maximises activities of both reverse transcriptase and AccuStart™ <i>Taq</i> DNA polymerase without compromising specificity of amplification. qScript™ One-Step SYBR® Green qRT-PCR kits are available and optimised for all real-time PCR instrument platforms including those requiring normalisation with ROX reference dye.

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