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Serum-free/low-protein medium, 1X with L-glutamine and 1 g/L BSA, without phenol red, Corning®

Catalog Number: (MDTC40-102-CV)

Supplier: Corning

Description: Corning® cellgro® COMPLETE formulation is based on a combination of 1:1 DMEM/F12, RPMI, and McCoy's 5A medium. This medium contains a proprietary mixture of selected trace elements and high molecular weight carbohydrates, extra vitamins, animal origin-free protein, and a small amount of high quality bovine serum albumin (1,0g/L). The Corning cellgro COMPLETE supports superior growth and viability over long-term passages in hybridomas, along with suspensions and adherent cell cultures.

UOM: 1 * 6 items

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Serum-free/low-protein medium, 1X with L-glutamine and 1 g/L BSA, Corning®

Catalog Number: (MDTC40-101-CV)

Supplier: Corning

Description: Corning® cellgro® COMPLETE formulation is based on a proprietary combination of 1:1 DMEM/F12, RPMI and McCoy's 5A medium. This medium contains a mixture of selected trace elements and high molecular weight carbohydrates, extra vitamins, animal origin-free protein, and a small amount of high quality bovine serum albumin (1,0 g/L). The Corning cellgro COMPLETE supports superior growth and viability over long-term passages in hybridomas, along with suspension and adherent cell cultures.

UOM: 1 * 6 items

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VWR®, Tube Rotator

Supplier: VWR Collection

Description: A unique design of rotator makes this unit suitable for a wide range of mixing applications in biochemistry, molecular biology and histochemistry. The rotisserie assemblies allow for mixing tubes vertically, horizontally, or any position in between, therefore, gentle mixing, using a horizontal position is easily accommodated for applications such as hybridisation. Alternatively rotation with vertically positioned tubes produces a tumbling motion suitable for mixing samples in blood tubes. The two halves of the rotisserie can be adjusted independently of each other to increase versatility and facilitate optimisation of mixing.

Incubator modules, Incubator 1000

Catalog Number: (444-0198)

Supplier: Heidolph Instruments GmbH & Co.KG

Description: Modular system for shaking, mixing and heating all in one. Any five kg shaker, e.g. Titramax 1000, Unimax 1010, Promax 1020, Duomax 1030 and Polymax 1040 can be combined with the incubator 1000 and a flat or high hood. The flat hood is recommended especially for microtitre plates, culture plates, small flasks, media bottles and Erlenmeyer flask up to 100 ml. The high hood is ideal for large flasks, media bottles and Erlenmeyer flasks up to 1000 ml. Hoods are made from transparent non-fogging PETG for easy viewing and immediate and convenient access to vessels. The heating module is ideal for use in molecular biology, bacteria cultivation and DNA analysis, it has temperature regulation via a PT100 sensor, and safety circuits prevent overheating.

UOM: 1 * 1 items

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Microplate shakers, MicroPlate Genie® and Multi-MicroPlate Genie®

Catalog Number: (444-0875)

Supplier: SCIENTIFIC INDUSTRIES ELMIS

Description: The high speed and small vortexing orbit of the mixers produces a true vortexing action so samples in each well of the microplate are mixed efficiently. Ideal for use in molecular biology applications and immunology laboratories.

UOM: 1 * 1 items

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Mag-Bind® Endo-Free plasmid 96 kit

Supplier: OMEGA BIOTEK

Description: The Mag-Bind® Endo-Free plasmid 96 kit combines the power of Mag-Bind® technology with the innovative ETR technology to deliver high quality endotoxin-free plasmid DNA in a high throughput format. By adding a quick endotoxin removal step, the Mag-Bind® Endo-Free plasmid 96 kit provides a flexible and fast solution for isolating endotoxin free plasmid on a small economical scale. The flexible system can be easily automated, thereby making it an ideal option for high throughput transfection screening as well as for other standard molecular biology techniques including restriction enzyme digestion.

Laboratory water makers, LAB-Q Ultra - Type I

Supplier: ION EXCHANGE INDIA

Description: LAB-Q Ultra - Type I is a multipurpose water maker. This water maker produces Type I ultrapure water and Type II pure water simultaneously. The produced water has very low total organic carbon (TOC) content, meeting the requirements of liquid chromatography and all spectroscopy methods, besides microbiological and molecular biology application. It also meets ISO 3696, Type I Standards. Dispensing of ultrapure water is configured on the front panel whereas pre water is dispensed from the storage tank. For feed water with higher level of TDS i.e. >500 ppm a customised pre treatment module can be designed prior to INDION Lab-Q Ultra.

E.Z.N.A.® Plasmid Midi kit

Supplier: OMEGA BIOTEK

Description: E.Z.N.A.® Plasmid Midi Kit offers a novel and reliable method of isolating up to 200 µg of plasmid DNA in less than 60 minutes. This kit combines HiBind® membrane technology with the time-tested consistency of alkaline SDS lysis of bacterial cells to deliver high-quality plasmid DNA. The DNase/RNase-free HiBind® DNA midi columns operate by eliminating time consuming phenol-chloroform extractions and alcohol precipitations. Plasmid purification using the E.Z.N.A.® Plasmid Midi Kit follows a simple bind-wash-elute procedure thereby making it possible for multiple samples to be processed. Although yields vary according to plasmid copy number, <i>E.coli </i>strain, and conditions of growth, 50 ml of an overnight culture in LB medium will typically produce 200 µg of high-copy number plasmid DNA. When working with low-copy number plasmids the starting culture volume can be increased up to 100 ml. High-quality DNA is ready for immediate use in routine molecular biology applications, such as automated fluorescent sequencing, restriction enzyme digestion, and transfection screening.

MSDS

3,3',5,5'-Tetramethylbenzidine (TMB) membrane peroxidase substrate Plus, liquid, Biotechnology Grade 1 component

Catalog Number: (K830-100ML)

Supplier: VWR Chemicals

Description: Ready to use sensitive substrate for the detection of horseradish peroxidase (HRP) activity suitable for ELISA and solution assays. Offers faster signal, enhanced sensitivity and the lowest possible background.

UOM: 1 * 100 mL

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Genomic DNA isolation from blood, E.Z.N.A.® SQ blood DNA kit

Supplier: OMEGA BIOTEK

Description: The E.Z.N.A.® SQ blood DNA kit provides a reliable method for the isolation of high molecular weight genomic DNA from fresh, frozen, or anti-coagulated whole blood. The method can also be used for preparation of genomic DNA from buffy coat, bone marrow or cultured cells. There is no need for phenol/chloroform extractions, and time consuming steps such as CsCl gradient ultracentrifugation are eliminated. DNA purified using the E.Z.N.A.® SQ blood DNA method is ready for downstream applications such as PCR, southern blotting and restriction enzyme digestion.