You Searched For: Hybridisation+Buffers

Supplier: MILLIPORE

Description: Optimized for the concentration and recovery of genomic DNA with SDS buffer. The low nonspecific binding characteristics of the membrane and the other device components, coupled with its medical-grade O-ring seal, allow the device to accommodate several wash steps with minimal sample loss.

New Product

Catalog Number: (VWRA06104119)

Supplier: VWR Collection

Description: DL-Dithiothreitol is used for stabilizing sulfhydryl containing enzymes. It is effective in sample buffers for reducing protein disu lfide bonds prior to SDS-PAGE. DTT can also be used for reducing the disulfide bridge of the cross-linker N,N'-bis(ac 1 * 25 g

UOM: 1 * 25 g

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Supplier: OMEGA BIOTEK

Description: The E.Z.N.A.® Insect DNA kit is designed for efficient recovery of genomic DNA up to 60 kb in size from insects, arthropods, roundworms, flatworms, invertebrates, as well as some plant tissue samples. The method is suitable for samples frozen or preserved in alcohol or DNE solution, and good results can be obtained with formalin preserved material. The procedure relies on the well established properties of the cationic detergent, cetyltrimethyl ammonium bromide (CTAB), in conjunction with the selective DNA binding of HiBind matrix. This allows salts, proteins and other contaminants to be removed to yield high quality genomic DNA suitable for downstream applications such as endonuclease digestion, thermal cycle amplification, and hybridisation techniques.

MSDS

Catalog Number: (216-1098)

Supplier: GRANT INSTRUMENTS

Description: All in one system with integrated pump designed for routine aspiration of the supernatent alcohol/buffer from the walls of microtubes during DNA/RNA purification and other macromolecule reprecipitation techniques. An ideal personal tool for independent operation away from an in-line lab vacuum supply.

UOM: 1 * 1 items

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Catalog Number: (N854-1.2ML)

Supplier: VWR Chemicals

Description: EZ-Vision® 1 kb DNA ladder is a pre-mixture of DNA marker, loading buffer and a proprietary fluorescent DNA visualisation dye that can be applied directly to the gel. This non toxic, non mutagenic dye eliminates the need for ethidium bromide staining. The 1 kb ladder consists of 10 double-stranded bands with molecular weights between 200 and 10 000 bp. The intensity of individual bands varies to facilitate easy identification.

UOM: 1 * 1,2 mL

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Supplier: Quantabio

Description: AccuStart™ II <i>Taq</i> DNA Polymerase is a high purity, recombinant <i>Taq</i> DNA polymerase preparation with high avidity monoclonal antibodies that bind the polymerase and keep it inactive prior to the initial PCR denaturation step. Upon heat activation (1 minute at 94 °C), the antibodies denature irreversibly, releasing fully active, unmodified <i>Taq</i> DNA polymerase. This enables specific and efficient primer extension with the convenience of room temperature reaction assembly. Non-specific extension of primers at low temperatures is a common cause of artifacts and poor sensitivity in PCR. The AccuStart™ II automatic hot-start enables specific and efficient primer extension in the PCR process with the added convenience of room temperature reaction assembly. The included 10X PCR Buffer II is a new optimised buffer that provides higher product yield, improved specificity, and enhanced multiplexing capability.

MSDS

Catalog Number: (700-0416)

Supplier: VWR Collection

Description: The kuroGEL semi-dry blotter provides great flexibility in a laboratory since it can be used for all types of blotting: Western, Southern and Northern. Blotting is fast and easy, and can typically be completed in 15 to 30 min. Set-up procedures are easy and economical with little buffer needed. The screw down lid adjusts to varying gel thicknesses and sizes, while the platinum coated electrodes provide uniform pressure ensuring even transfers.

UOM: 1 * 1 items

Sale

Supplier: VWR Collection

Description: PerfectBlue S and M tank electroblotters are designed to rapidly transfer nucleic acid or protein fragments from up to four polyacrylamide gels at a time to nitrocellulose, nylon or PVDF membranes. The colour coded cassettes allow for easy assembly of transfer sandwich and error-free transfer. The large buffer capacity and integral cooling allow extended transfer times and high voltage/high current operation for the transfer of high molecular weight proteins.

Supplier: VWR Collection

Description: Designed for the preparation of buffers, tissue culture media, microbiological media and other biological fluids. These vacuum filtration systems feature large diameter high performance PES membranes for guaranteed performance and maximum throughput. The full assembly includes a filtration unit and a receiver bottle with an ergonomic shape, moulded finger grips and a patented bottle cap. The centrifuge tube and funnel system allows the user to filter fluids directly into a 15 or 50 ml tube.

Supplier: MILLIPORE

Description: The Amicon® Pro device streamlines the affinity purification process by providing a single adaptable centrifugation unit capable of performing all steps in the affinity purification process. The device combines affinity-based spin column purification with downstream sample concentration and buffer exchange, eliminating the need for multiple sample transfers, thereby minimising protein loss.

New Product

Catalog Number: (R6870-00)

Supplier: OMEGA BIOTEK

Description: The E.Z.N.A.® Yeast RNA kit is designed for isolation of high quality total RNA from a wide variety of yeast species. Up to 5×10⁷ log-phase yeast cultures can be processed for each column. Using an enzyme/beads-beating procedure to efficiently break the cell, combined with an optimised cell lysis buffer, the E.Z.N.A.® Yeast RNA kit can purify high-quality of total cellular RNA from various yeast strains.

UOM: 1 * 5 Tests

Certificates Sale

Supplier: BIOWEST

Description: In 1976, Guilbert and Iscove demonstrated that precursor cells of erythrocytes and macrophages could be cultured in a reduced-serum medium supplemented with albumin, transferrin, lecithin, and selenium. Iscove's medium is a modification of Dulbecco's Modified Eagle's Medium (DME) containing selenium, additional amino acids and vitamins, sodium pyruvate, HEPES buffer, and potassium nitrate instead of ferric nitrate. Further studies have demonstrated that Iscove's medium will support murine B lymphocytes, haemopoietic tissue from bone marrow, B-cells stimulated with lipopolysaccharide, T-lymphocytes, and a variety of hybrid cells.

MSDS Certificates

Supplier: MILLIPORE

Description: These centrifugal filter units can be used for a number of applications: concentration of biological samples containing antigens, antibodies, enzymes, nucleic acids, or microorganisms; purification of macromolecular components found in tissue culture extracts or cell lysates and protein removal prior to HPLC; concentration of dilute or pre-purified proteins from column eluents; desalting, buffer exchange and protein dialysis; size exclusion and protein fractionation.

New Product

Supplier: MILLIPORE

Description: These centrifugal filter units can be used for a number of applications: concentration of biological samples containing antigens, antibodies, enzymes, nucleic acids, or microorganisms; purification of macromolecular components found in tissue culture extracts or cell lysates and protein removal prior to HPLS; concentration of dilute or pre-purified proteins from column eluents; desalting, buffer exchange and protein dialysis; size exclusion and protein fractionation.

New Product

Supplier: VWR Collection

Description: VCP 80 and VCP 130 can be used as vacuum and pressure pumps, and set a standard for efficient vacuum use. Use these pumps as an affordable vacuuming application where corrosion resistance is not required. These pumps can be used for vacuum filtration of aqueous solutions, or aqueous fumes including buffers, but are not recommended for pumping acidic, basic or organic fumes. These VWR® Collection vacuum/pressure pumps are designed for continuous unattended operation, and have two dial gauges with manual regulators that enable you to adjust and easily monitor the required vacuum or pressure level.

Supplier: Quantabio

Description: FastMixes include buffers and stabilisers optimised for maximum PCR efficiency, reproducibility, and sensitivity with TaqMan® and TaqMan® MGB probe chemistries. A key component of PerfeCTa® qPCR FastMixes is AccuFast™ <i>Taq</i> DNA polymerase that enables instant activation and rapid polymerisation kinetics. Upon heat activation (20 seconds at 95 °C), the antibodies irreversibly denature releasing fully active, unmodified <i>Taq</i> DNA polymerase. This promotes specific and efficient primer extension with the convenience of room temperature reaction assembly. FastMixes deliver maximum PCR efficiency, sensitivity, specificity, and a robust fluorescent signal with either fast or conventional cycling conditions.

MSDS