You Searched For: Extraction+Buffers

Catalog Number: (M334-100ML)

Supplier: VWR Chemicals

Description: Detergent-free buffer for isolation of proteins from tissue culture cells. Cell lysis and the subsequent isolation of the crude cytoplasmic fraction completed in less than 30 m.

UOM: 1 * 100 mL

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Supplier: Avantor

Description: J.T. Baker® Cell Lysis Solution. Optimized multi-component, ready-to-use solution for extraction and recovery of AAV particles from HEK293 or SF9 cells. The product offers quantitative recovery through efficient cell lysis and protection of vector particles against shear stress. The solution is formulated with non-ionic detergents which are octyl phenol ethoxylate free.

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Supplier: Avantor

Description: J.T.Baker® Cell Lysis Solution Biotech Reagent is a cGMP pre-formulated and ready to use detergent optimized for the extraction and recovery of AAV particles from producer cell lines (HEK293 parental and suspension lines, SF9). J.T.Baker® Cell Lysis Solution is a proprietary blend of nonionic surfactants, free of octyl phenol ethoxylate, which demonstrates comparable performance industry standard detergents while addressing environmental concerns associated with REACH and downstream clearance concerns. Designed for Gene Therapy workflows, this formulation is easy to use and handle as it is ready-to-use, has low viscosity, does not require cold temperature shipping or storage, and is available in sizes to support processes from development to large scale commercial batches. Detail description provided in technical literature.

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Supplier: OMEGA BIOTEK

Description: The E.Z.N.A.® RNALock Stabilizer Reagent is an innovative reagent which rapidly lyses cells and tissues to inactivate RNases at room temperature. Simply submerge the samples, such as cell cultures, animal tissues, or white blood cells into the RNALock Stabilizer Reagent. The intact cellular RNA will be preserved even at elevated temperature. Samples can be stored in this reagent in excess of 12 months at −20 °C.

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Supplier: VWR Chemicals

Description: Phenol:Chloroform pH 6.7/8.0 is premixed with isoamyl alcohol (25:24:1). A phenol:chloroform mixture is ideal for the extraction of protein from DNA preparations. Packaged at pH 6.7, this product is accompanied by a separate alkaline buffer which allows the researcher to increase the pH to 8.0. Doubly distilled, high purity phenol packaged under nitrogen. Safer, easier alternative to crystalline phenol.

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Supplier: OMEGA BIOTEK

Description: The Mag-Bind® Plant DNA DS 96 Kit is designed for difficult samples (DS) which are hard to lyse or contain high amounts of polysaccharides and polyphenols. The system uses a CTAB-based lysis buffer which does not require organic solvents. The proprietary binding system prevents inhibitors from binding to the magnetic beads. The wash buffer system further removes polysaccharides, phenolic compounds and enzyme inhibitors from plant tissue lysate. An optional rebinding step is included with the kit but most samples do not require the additional treatment.

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Catalog Number: (R6870-00)

Supplier: OMEGA BIOTEK

Description: The E.Z.N.A.® Yeast RNA kit is designed for isolation of high quality total RNA from a wide variety of yeast species. Up to 5×10⁷ log-phase yeast cultures can be processed for each column. Using an enzyme/beads-beating procedure to efficiently break the cell, combined with an optimised cell lysis buffer, the E.Z.N.A.® Yeast RNA kit can purify high-quality of total cellular RNA from various yeast strains.

UOM: 1 * 5 Tests

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Catalog Number: (M1128-01)

Supplier: OMEGA BIOTEK

Description: The Mag-Bind® Plant DNA 96 plus kit allows rapid and reliable isolation of high-quality genomic DNA from a wide variety of difficult to lyse and high polysaccharide plant species and tissues. The system utilises a CTAB-based lysis buffer without the need for a chloroform or other organic solvent steps. The proprietary wash buffer systems eliminate polysaccharides, phenolic compounds, and enzyme inhibitors from plant tissue lysates. This kit is designed for manual or fully automated high throughput preparation of genomic, chloroplast, and mitochondrial DNA. The Mag-Bind® Plant DNA plus kit also provides greater flexibility than silica plate based systems as partial plates can be used and can be scaled up to a 24-well format with the appropriate magnetic stand. The streamlined protocol can be easily adapted on most robotic liquid handling platforms. Purified DNA is suitable for PCR, qPCR, restriction digestion, hybridisation applications, as well as more demanding applications, such as RAPD.

UOM: 1 * 1 KIT

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Supplier: OMEGA BIOTEK

Description: E.Z.N.A.® MicroElute® Genomic DNA kit provides a rapid and easy method for the isolation of genomic DNA from small sample volumes and sizes for consistent PCR and analytical test results. Using the reversible binding properties of a silica-based material, the speed of mini-column spin technology, and specifically formulated buffers, the system allows the purification of genomic DNA up to 60 kb. Samples are first lysed under denaturing conditions and then applied to the HiBind MicroElute® Column to bind DNA, while cellular debris, haemoglobin, and other proteins are effectively washed away. High quality DNA can be eluted with 10 to 15 µl sterile deionised water or elution buffer from the MicroElute® column.

Supplier: OMEGA BIOTEK

Description: Elution buffer, Omega Bio-Tek

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Supplier: OMEGA BIOTEK

Description: Modified from the E.Z.N.A.® Plant RNA kit, the E.Z.N.A.® Fungal RNA kit uses an improved buffer system that is more efficient for fungal samples. This kit provides convenient and rapid methods for the isolation of total RNA from a variety of fungal species. Rather, the method involves a simple and rapid precipitation step for removal of much of the polysaccharides and phenolic compounds commonly found in fungal tissues. In combination with HiBind® RNA spin columns, this method permits purification of high quality RNA from as much as 200 mg tissue. The system is also efficient enough to purify total RNA from as little as 10 mg of tissue or 100 cells. Purified RNA can be used for many downstream applications such as RT-PCR, northern blotting, Poly(A)+ mRNA purification, and differential display.

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Supplier: OMEGA BIOTEK

Description: BL buffer, Omega Bio-Tek

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Supplier: APPLICHEM LIFESCIENCE

Description: Cationic detergent cetyltrimethylammonium bromide (CTAB) is used to liberate and complex with total cellular nucleic acids.

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Supplier: OMEGA BIOTEK

Description: The E-Z 96® Total RNA Kit is designed for isolation of total cellular RNA from up to 5×10⁶ cultured cells or soft tissues. This kit can process single or multiple samples in less than 60 minutes. Utilising HiBind® silica plate technology, the need for phenol/chloroform extractions, CsCl gradient ultracentrifugation, and precipitation with isopropanol or LiCl are eliminated. Samples are lysed in a denaturing lysis buffer which inactivates RNases. Binding conditions are adjusted and the lysate is transferred to a 96-well HiBind® RNA Plate where the RNA is purified via three wash steps. High quality RNA is eluted in RNase-free water. RNA purified using the E-Z 96® Total RNA method is ready for applications such as RT-PCR, qPCR, differential display, microarrays, and other downstream applications.

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Catalog Number: (CSPL-1000)

Supplier: OMEGA BIOTEK

Description: Component of the Mag-Bind® plant DNA 96 plus kit.

UOM: 1 * 1 items

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Supplier: OMEGA BIOTEK

Description: The Mag-Bind® PX blood RNA kit allows for the isolation of total RNA from 2,5 ml of whole blood that has been stored in PAXgene® Blood RNA tubes. Stabilised blood samples are first spun down and the nucleic acid pellets are washed and recollected. After washing, the nucleic acid pellet is dissolved into a specially formulated resuspension buffer. At this point the samples can be transferred to 96-well plates for processing in robotic liquid handlers and magnetic processors. High quality intact RNA can be isolated in less than 90 minutes.