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Formalin-fixed, paraffin-embedded (FFPE) tissue RNA kits, E.Z.N.A®

Supplier: OMEGA BIOTEK
Description: Selected protease digestion releases microgram amounts of RNA from FFPE samples. The purified nucleic acids, although highly fragmented, are suitable for a variety of downstream genomic and gene expression analyses. E.Z.N.A® spin column-based kits are ideal for low throughput applications while Mag-Bind® magnetic beads-based kits are designed specifically for high throughput users with automation capability.

MSDS Certificates

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MicroElute RNA clean up kit, E.Z.N.A.®

Supplier: OMEGA BIOTEK
Description: The MicroElute RNA clean-up kit is designed to purify and concentrate RNA (from 50 μg down to picogram amounts) from enzymatic reactions or for desalting RNA samples (e.g. following phenol extraction and ethanol precipitation). The procedure uses lower elution volumes, making it well suited for applications require highly concentrated RNA sample.

MSDS Certificates

Image Unavailable-M6958-00

E.Z.N.A.® Formalin-Fixed, Paraffin-Embedded (FFPE) Tissue DNA Kits

Supplier: OMEGA BIOTEK
Description: Selected protease digestion releases microgram amounts of DNA from FFPE samples.

MSDS

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DNA mini columns for genomic/plasmid DNA extraction, Hi-Bind®

Supplier: OMEGA BIOTEK
Description: Hi-Bind® DNA mini columns are silica glass fibre columns that can bind up to 100 μg of genomic DNA or 35 μg of plasmid DNA per prep. Columns feature an attached lid and a vacuum luer for processing with vacuum manifolds and by centrifugation.

Product Image-R6830-02

RNA-Solv® reagent

Catalog Number: (R6830-02)
Supplier: OMEGA BIOTEK
Description: RNA-Solv® Reagent is a one reagent system for the isolation of total RNA from cells and tissues. The reagent, a single-phase solution consisting of phenol and guanidine isothiocyanate, is a modification of the single-step RNA isolation method developed by Chomczynski and Sacchi. The sample is homogenised and lysed in RNA-Solv® Reagent, which maintains the integrity of the RNA while disrupting and denaturing endogenous RNases and other cellular components. Extraction of the lysate with chloroform further denatures proteins and separates the mixture into an organic and an aqueous phase. RNA remains exclusively in the aqueous phase, and is subsequently recovered by isopropanol.
UOM: 1 * 200 mL

MSDS Certificates


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Bacterial DNA kit, E.Z.N.A.®

Supplier: OMEGA BIOTEK
Description: The E.Z.N.A.® bacterial DNA kit allows the rapid and reliable isolation of high quality total cellular DNA from a wide variety of bacterial species. This kit uses optimised lysis condition and up to 1×10⁹ bacterial cells can be processed for each column. There are no organic extractions, thus reducing plastic waste and hands on time to allow multiple samples to be processed in parallel. Bacterial cells are grown to log-phase and harvested. The cell wall is removed by lysozyme digestion and bead beating, followed by protease digestion. Following lysis, binding conditions are adjusted and the sample is applied to a HiBind DNA spin-column. Two rapid wash steps remove trace salts and protein contaminants, and DNA is finally eluted in water or elution buffer. Purified DNA can be directly used in downstream applications without the need for further purification.

MSDS

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E.Z.N.A.® miRNA isolation kit

Supplier: OMEGA BIOTEK
Description: The E.Z.N.A.® miRNA isolation kit uses a rapid procedure to isolate small RNAs, such as micro RNA (miRNA), small interfering RNA (siRNA), and small nuclear RNA (snRNA), from wide range of tissues and cells. The fast and efficient silica membrane based method isolates total RNA ranging in size from kilo based down to decamers. The kit also provides reagents and a procedure to enrich the population of RNAs that are 200 bases and smaller, which enhances the sensitivity of small RNA detection by solution hybridisation, Northern analysis, and other methods.

MSDS

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DNA plates for genomic/plasmid DNA extraction, E-Z® 96

Supplier: OMEGA BIOTEK
Description: E-Z® 96 DNA plates are silica glass fibre plates can bind up to 50 μg of genomic DNA or 20 μg of plasmid DNA per prep. Each well has a capacity of 1 ml and the plates are semi skirted. E-Z 96 DNA plates can be processed in vacuum manifolds and/or by centrifugation.

Image Unavailable-D6950-01

E.Z.N.A.® Endo-Free plasmid mini kit II

Supplier: OMEGA BIOTEK
Description: Plasmid isolated with traditional purification procedures normally contain high levels of endotoxins (also known as lipopolysaccharides or LPS) that can significantly interfere with transfection experiments downstream. The E.Z.N.A.® Endo-Free plasmid mini kit II integrates an efficient endotoxin removal step into the plasmid purification procedure to produce high-quality transfection grade (<0,1 EU/µg) plasmid for efficient transfection. The bacterial cells are lysed using the alkaline-SDS lysis method. The cleared cell lysate is then treated with ETR reagent to efficiently remove the endotoxins. After adjusting the binding condition, the cell lysate is applied into the HiBind® DNA column and purified DNA is eluted from the column membrane.

Product Image-A9742.0002

Thermal cycler, validation kits

Catalog Number: (A9742.0002)
Supplier: PanReac AppliChem
Description: False negative or non-specific PCR results are highly critical and might be caused by a defect heating element (peltier element) of the PCR cycler. Control of the reaction temperature is therefore essential. The kit provides a temperature sensitive PCR reaction to monitor inconsistencies in the temperature range of the thermal cycler.
UOM: 1 * 2 Tests

Certificates


Product Image-391-2007

Bacterial cell spreaders, SP Bel-Art

Supplier: Bel-Art Products, a Part of SP
Description: For spreading cells onto the surface of an agar plate to form individual colonies. These stainless spreaders have a smooth triangular end with a straight spreading bar.

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Dnase

Supplier: OMEGA BIOTEK
Description: For DNase digestion during RNA purification. The RNase-free DNase set provides a rapid and efficient on-column digestion of DNA during RNA purification from a wide variety of sample sources using E.Z.N.A.® RNA kits. The DNases can be efficiently removed in subsequent steps. Generally, DNase digestion is not required for RNA purified with E.Z.N.A.® RNA kits since the silica-membrance, spin-column technology efficiently removes the majority of the DNA without DNase treatment. However, complete DNA removal may be necessary for certain RNA applications that are sensitive to very small amounts of DNA.

MSDS Certificates

Product Image-489-0091

Gamma radiation sterilisation indicator labels

Supplier: CROSSTEX
Description: Self-adhesive, non-toxic, chemical process indicators that change from yellow to red upon exposure to gamma or electron beam radiation. Labels are coated with a UV blocking agent for protection from exposure to UV light. A minimum of 15 kGy irradiation is recommended for sufficient end-point colour development. At greater dosage levels, the radiation indicators exhibit a darker shade of red.

MSDS Certificates

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Tissue direct PCR kits

Catalog Number: (TQ2310-00)
Supplier: OMEGA BIOTEK
Description: The tissue direct PCR kit is a buffer system for direct PCR from animal tissue and cultured cells that effectively lyses the tissue and neutralises inhibitors. The kit requires as little as 5 to 10 mg of tissue.
UOM: 1 * 20 Tests
Product Image-444-0336

Combined centrifuge/vortex mixers, PCV-2400 / PCV-6000

Catalog Number: (444-0336)
Supplier: GRANT INSTRUMENTS
Description: Combi-spin™ (PCV-2400) and Multi-spin™ (PCV-6000) provide a simple and effective means of carrying out combined or independent centrifuge and mixing applications in 0,5/1,5/2,0 ml microtubes and 0,2 ml strips. They can also be used for independent centrifuge and vortex mixing applications. All three models aid the sample preparation process for many procedures, including PCR, gel electrophoresis and enzyme restrictions.
UOM: 1 * 1 items
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BeneSphera™, ELISA Kit, HIV-1/2

Catalog Number: (BENP15000992)
Supplier: BENESPHERA
Description: BeneSphera™ HIV 1/2 is an <i>in vitro</i> enzyme immunoassay for the qualitative determination of antibodies to HIV-1 and HIV-2 in Human Serum or Plasma.
UOM: 1 * 96 Tests
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Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us at 1-800-932-5000.
This product is marked as restricted and can only be purchased by approved Shipping Accounts. If you need further assistance, email VWR Regulatory Department at eurega_services@eu.vwr.com
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The original product is no longer available. The replacement shown is available.
This product is no longer available. Alternatives may be available by searching with the VWR Catalog Number listed above. If you need further assistance, please call VWR Customer Service at 1-800-932-5000.
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