You Searched For: End-point+PCR+Enzymes+and+Kits

Supplier: Quantabio

Description: The AccuStart™ II Mouse Genotyping kit is designed for fast and easy preparation of PCR-ready DNA extracts and endpoint PCR analysis from tissues such as tail snips and ear punches commonly used for genotyping knockout and transgenic animals. The kit combines Extracta™ DNA Prep for PCR - Tissue with AccuStart™ II GelTrack™ PCR SuperMix in a single kit.

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Supplier: Quantabio

Description: AccuStart™ II PCR SuperMix is a 2X concentrated, ready-to-use reaction cocktail for routine PCR amplification of DNA fragments up to 4 kb. It contains all components, except primers and template. AccuStart™ II PCR SuperMix simplifies reaction assembly, improves assay reproducibility, and reduces the risk of contamination. A key component is AccuStart™ II Taq DNA polymerase which contains monoclonal antibodies that bind to the polymerase and keep it inactive prior to the initial PCR denaturation step. Upon heat activation (1 minute at 94 °C), the antibodies denature irreversibly, releasing fully active, unmodified Taq DNA polymerase. This enables specific and efficient primer extension with the convenience of room temperature reaction assembly.

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Catalog Number: (1B1409-100RXN)

Supplier: VWR Chemicals

Description: Hot Start PCR-To-Gel Taq Master Mix, 2X is a ready to use reaction cocktail that contains all required components, except primers and template DNA, for routine PCR amplification of DNA fragments up to 4 kb.

UOM: 1 * 1 KIT

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Supplier: Quantabio

Description: AccuStart™ II GelTrack PCR SuperMix is a 2X concentrated, ready-to-use reaction cocktail for routine PCR amplification of DNA fragments up to 4 kb followed by analysis on agarose gels. It contains all components, except primers and template. AccuStart™ II GelTrack PCR SuperMix simplifies reaction assembly, improves assay reproducibility, and reduces the risk of contamination. The supermix includes electrophoresis tracking dyes that migrate at approximately 4 kb and 50 bp to allow direct loading of PCR product on agarose gels following amplification. AccuStart™ II <i>Taq</i> DNA polymerase in the master mix is inactivated with monoclonal antibodies that bind the polymerase and keep it inactive prior to the initial PCR denaturation step. Upon heat activation (1 minute at 94 °C), the antibodies denature irreversibly, releasing fully active, unmodified <i>Taq</i> DNA polymerase. This enables specific and efficient primer extension with the convenience of room temperature reaction assembly.

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Supplier: VWR Chemicals

Description: An optimal buffer system is critical for the performance of successful PCR. VWR® Taq DNA Polymerase kits generally include two different buffers, Key Buffer and Extra Buffer, which are suited for different PCR needs. All buffers contain Tris and 15 mM MgCl₂ (1,5 mM MgCl₂ final concentration). Additional MgCl₂ for easy optimisation is included in a separate vial.

Catalog Number: (VWRA06105500)

Supplier: VWR Collection

Description: VWR® Delivered High quality Reagents & biochemicals, combined with innovative products that gives optimum results , save time & simplify protocols

UOM: 1 * 200 Tests

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Catalog Number: (N806-2X1.25ML)

Supplier: VWR Chemicals

Description: Ready PCR Mix, 2X offers a single-step procedure for performing PCR reactions followed by analysis on agarose gels without the addition of loading buffer. All components for assembly and performance of PCR reactions as well as loading and visualisation of PCR products on agarose gels are included. The user supplies primer and template DNA.

UOM: 1 * 1 KIT

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Supplier: Quantabio

Description: AccuStart™ II PCR ToughMix® is a 2X concentrated ready to use reaction cocktail for PCR amplification of DNA templates that overcomes many known inhibitors of PCR often present in crude samples extracted from environmental specimens, plant tissues, or animal tissues. It contains all components, except primers and template.

Supplier: VWR Chemicals

Description: VWR® TEMPase Hot Start DNA Polymerases are highly stable polymerases, featuring higher specificity, superior sensitivity and greater yields compared to standard DNA polymerases. These features make them well suited for the detection of low abundance targets. Other uses include screening, amplification of GC-rich sequences, multiplex PCR, direct PCR and qPCR.

Catalog Number: (733-2873)

Supplier: VWR Chemicals

Description: Highly concentrated Taq DNA polymerase glycerol free 50 U/µl is ideal for industrial use and manufactures of diagnostic PCR kits.

UOM: 1 * 1 KIT

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Supplier: VWR Chemicals

Description: VWR® <i>Taq</i> DNA Polymerase Master Mix is a ready to use 1,1X or 2X reaction mix. Simply add primers, template and water to carry out primer extensions and other molecular biology applications.

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Catalog Number: (N224-500U)

Supplier: VWR Chemicals

Description: <i>Taq</i> DNA Polymerase is ideal for general PCR and primer extension reactions to amplify DNA templates with high sensitivity. PCR can be initiated from as few as 6 primed templates. Simply add 0,5 µl of <i> Taq</i> DNA Polymerase to a 25 µl PCR reaction.

UOM: 1 * 500 items

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Supplier: VWR Chemicals

Description: VWR® Red <i>Taq</i> DNA Polymerase Master Mix, which also contains an inert red dye, can be directly loaded onto an agarose gel without addition of electrophoresis loading buffers.

Supplier: VWR Chemicals

Description: VWR® TEMPase Hot Start DNA Polymerases are highly stable polymerases, featuring higher specificity, superior sensitivity and greater yields compared to standard DNA polymerases. These features make them well suited for the detection of low abundance targets. Other uses include screening, amplification of GC-rich sequences, multiplex PCR, direct PCR and qPCR. A glycerol-free TEMPase Hot Start DNA Polymerase is also available for automation and freeze drying.

Supplier: Quantabio

Description: Superior speed and inhibitor tolerance.

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Supplier: VWR Chemicals

Description: Red Taq DNA Polymerase is a blend of Taq DNA polymerase combined with an inert red dye. The dye enables quick visual recognition of reactions to which enzyme has been added, as well as confirmation of complete mixing.

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