You Searched For: Dehydrated+Media+for+Microbiology

Supplier: BIOWEST

Description: CRML was originally formulated for promoting strain L (Earle) cells under serum-free conditions. These media are also known for its ability to sustain cloning of monkey kidney cells and for growth of many other mammalian cell lines when supplemented with horse or calf serum.

Supplier: BIOWEST

Description: The long term cultivation of cells requires the addition of serum. When properly supplemented, Medium 199 has broad species applicability, particularly for cultivation of non-transformed cells. It is widely used in virology, vaccine production and<i> in vitro</i> cultivation of primary explants of mouse pancreatic epithelial and rat lens tissues.

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Supplier: BIOWEST

Description: Ham's F10 medium is intended to support the growth of Chinese Hamster Ovary cells under serum-free conditions and other mammalian cell types with serum supplementation. It is a popular medium for growth of fastidious cell lines.

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Catalog Number: (MDTC25-022-CI)

Supplier: Corning

Description: Trace Elements B Solution has the same composition as Cleveland's Trace Element I.

UOM: 1 * 100 mL

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Supplier: BIOWEST

Description: Dulbecco's Modified Eagle Medium (DMEM) is a modification of Basal Medium Eagle (BME) which contains approximately four times as much of the vitamins and amino acids, and two to four times as much glucose (depending on the version) as the original formulation.

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Catalog Number: (L0138-500)

Supplier: BIOWEST

Description: Ham's F14 medium was developed from Ham's F12 medium, which was originally developed for the serum-free clonal growth of Chinese Hamster Ovary (CHO) cells, lung cells and mouse L-cells. Ham's F14 contains a double concentration of amino acids compared to the Ham's F12 medium. This product is also supplemented with calcium chloride and ascorbic acid.

UOM: 1 * 500 mL

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Catalog Number: (27357.232)

Supplier: VWR Chemicals

Description: This product has a specific odour, it may be colourless to light yellow. The yellowing does not affect adversely the clarifying efficiency of the reagent.

UOM: 1 * 250 mL

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Supplier: BIOWEST

Description: Glasgow MEM (GMEM) was originally formulated for growing BHK 21 cells. The original formulation is an Eagle's Basal Medium modified to contain two times the normal concentration of amino acids and vitamins. It is intended for use with adherent cell lines.

Supplier: BIOWEST

Description: Minimum Essential Medium (MEM) with Earle's Balanced Salts is a modification of Eagle's earlier medium, Basal Medium Eagle (BME), containing higher concentrations of the essential nutrients. These media have demonstrated the ability to support a variety of normal and transformed cells in culture and contain Earle's Balanced Salts, which make them suitable for use in atmospheres charged with CO₂ gas.

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Supplier: BIOWEST

Description: RPMI are general purpose enriched media with extensive applications for a broad spectrum of mammalian and hybridoma cells, including human myeloma, mouse hybridoma, human leukocytes, and B and T lymphocytes. It was originally formulated for suspension cultures and monolayer culture of human leukaemia cells.

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Supplier: BIOWEST

Description: In 1976, Guilbert and Iscove demonstrated that precursor cells of erythrocytes and macrophages could be cultured in a reduced-serum medium supplemented with albumin, transferrin, lecithin, and selenium. Iscove's medium is a modification of Dulbecco's Modified Eagle's Medium (DME) containing selenium, additional amino acids and vitamins, sodium pyruvate, HEPES buffer, and potassium nitrate instead of ferric nitrate. Further studies have demonstrated that Iscove's medium will support murine B lymphocytes, haemopoietic tissue from bone marrow, B-cells stimulated with lipopolysaccharide, T-lymphocytes, and a variety of hybrid cells.

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Catalog Number: (MDTC25-023-CI)

Supplier: Corning

Description: Trace Elements C Solution has the same composition as Cleveland's Trace Elements I, and contains the trace elements found in the serum-free medium MCDB301.

UOM: 1 * 100 mL

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Supplier: VWR Chemicals

Description: Ficoll® 400 is a neutral, hydrophilic polymer of sucrose, which readily dissolves in aqueous solutions.

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Supplier: VWR Collection

Description: These tank liners are single-use bags with an open top, designed to fit in cylindrical vessels.

Supplier: BIOWEST

Description: Studies to determine the nutritional requirements of many cells have been in progress since Eagle's first reports. The major essential nutrients were identified, and work became focussed on the media requirements of individual cell types. Many media designed for these purposes are now available. Among the first of these media, developed initially to study hormonal requirements of cells in culture, was a mixture of DMEM and Ham's F12 medium, known as DMEM-Ham's F12.

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Supplier: BIOWEST

Description: Ham's F12 was originally developed for the serum-free clonal growth of Chinese Hamster Ovary (CHO) cells, lung cells and mouse L-cells. It is frequently used with dialysed serum, hormones, selenium and other supplements for serum-free cultures. It is the medium of choice for supporting the growth of cells of rodent origin, particularly rabbit and rat and has proved to be an excellent cloning medium for the culture of myeloma and hybrid cells.

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