You Searched For: Culture+Flasks

Supplier: BIOWEST

Description: The long term cultivation of cells requires the addition of serum. When properly supplemented, Medium 199 has broad species applicability, particularly for cultivation of non-transformed cells. It is widely used in virology, vaccine production and<i> in vitro</i> cultivation of primary explants of mouse pancreatic epithelial and rat lens tissues.

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Supplier: BIOWEST

Description: Glasgow MEM (GMEM) was originally formulated for growing BHK 21 cells. The original formulation is an Eagle's Basal Medium modified to contain two times the normal concentration of amino acids and vitamins. It is intended for use with adherent cell lines.

Catalog Number: (L0042-500)

Supplier: BIOWEST

Description: Basal Medium Eagle is principally used for monolayer diploid or primary cell cultures. It is the simplest of the basal media with all essential components for growth. BME ideally supports cell lines such as HeLa, L-cells and primary mammalian fibroblasts. BME has also successfully been used for the growth of human transformed cell lines (particularly WI-38 and MRC-5).

UOM: 1 * 500 mL

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Supplier: BIOWEST

Description: Studies to determine the nutritional requirements of many cells have been in progress since Eagle's first reports. The major essential nutrients were identified, and work became focussed on the media requirements of individual cell types. Many media designed for these purposes are now available. Among the first of these media, developed initially to study hormonal requirements of cells in culture, was a mixture of DMEM and Ham's F12 medium, known as DMEM-Ham's F12.

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Supplier: BIOWEST

Description: Trypsin is a porcine pancreas-derived enzyme that is commonly used for the dissociation and disaggregation of anchorage-dependent mammalian cells and tissues. The concentration of trypsin necessary to dislodge the cells from their substrate depends on the sensitivity of the cells.

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Supplier: BIOWEST

Description: L-glutamine is an essential amino acid required by virtually all mammalian and insect cells grown in culture. It is a crucial component of many cell culture media and serves as a major energy source for cells in culture. L-glutamine is very stable as a dry powder and as a frozen solution. However, in liquid media or stock solutions, L-glutamine can degrade relatively rapidly. L-glutamine is also more labile in cell culture solution than other amino acids.

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Catalog Number: (L0138-500)

Supplier: BIOWEST

Description: Ham's F14 medium was developed from Ham's F12 medium, which was originally developed for the serum-free clonal growth of Chinese Hamster Ovary (CHO) cells, lung cells and mouse L-cells. Ham's F14 contains a double concentration of amino acids compared to the Ham's F12 medium. This product is also supplemented with calcium chloride and ascorbic acid.

UOM: 1 * 500 mL

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Catalog Number: (391-0690)

Supplier: DWK LIFE SCIENCES

Description: DURAN®, borosilicate glass 3.3, clear.

UOM: 1 * 1 items

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Supplier: DWK LIFE SCIENCES

Description: Soda-lime glass with straight rim.

Supplier: UPM

Description: <B>GrowDex® Hydrogel</B> is a bio-friendly hydrogel extracted from birch and sourced from sustainable and responsibly managed forests. GrowDex® is a ready to use hydrogel that mimics the extracellular matrix (ECM), supporting cell growth and differentiation with consistent results. The versatility of GrowDex® has seen it used in numerous applications ranging from 3D cell culture and organ-on-a-chip models, through to drug release studies and 3D printing. GrowDex® is ideal for these and other applications due to its unique properties.

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Supplier: BIOWEST

Description: In 1976, Guilbert and Iscove demonstrated that precursor cells of erythrocytes and macrophages could be cultured in a reduced-serum medium supplemented with albumin, transferrin, lecithin, and selenium. Iscove's medium is a modification of Dulbecco's Modified Eagle's Medium (DME) containing selenium, additional amino acids and vitamins, sodium pyruvate, HEPES buffer, and potassium nitrate instead of ferric nitrate. Further studies have demonstrated that Iscove's medium will support murine B lymphocytes, haemopoietic tissue from bone marrow, B-cells stimulated with lipopolysaccharide, T-lymphocytes, and a variety of hybrid cells.

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Supplier: DWK LIFE SCIENCES

Description: Soda-lime glass with DIN thread and PP screw cap.

Supplier: Greiner Bio-One

Description: Microplates with a modified culture surface to optimise cell characteristics and functions. The improved adherence and increased growth rate improve and accelerate cell proliferation. Additionally, the Advanced TC™ surface facilitates uniform and consistent cell adherence, increases throughput and reduces cell loss, for example during automated washing stages. Free of detectable DNase, RNase and human DNA.

Supplier: DWK LIFE SCIENCES

Description: DURAN®, borosilicate glass 3.3, clear, with DIN thread and red PBT screw caps with PTFE coated gaskets.

Catalog Number: (P5648-10GR)

Supplier: BIOWEST

Description: Phenol red is used as a pH indicator in cell culture. Its colour exhibits a gradual transition from yellow to red over the pH range 6,8 to 8,2. Above pH 8,2, phenol red turns a bright pink (fuchsia) colour.

UOM: 1 * 10 g

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Supplier: VWR Chemicals

Description: VWR® dehydrated culture media that has been granulated offers both convenience and safety benefits. The correct dissolution of culture media is critical to achieving the best performance. Granulated culture media saves time in the preparation of ready to use culture media. In addition, granulation reduces dust generation, so handling the product is more pleasant and safer for the user, making it a much cleaner and safer working environment.

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