You Searched For: Cell+Culture+Supplements+and+Additives

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VWR® Egg Yolk Emulsion, Sterile

Catalog Number: (536262U)

Supplier: VWR Chemicals

Description: Egg yolk emulsion, sterile, VWR®

UOM: 1 * 100 mL

MSDS Certificates Sale

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Cell culture growth supplement, Trace Elements A, Corning®

Catalog Number: (MDTC25-021-CI)

Supplier: Corning

Description: Trace Elements A provides copper, zinc, iron, and selenium

UOM: 1 * 100 mL

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Trypsin

Supplier: BIOWEST

Description: Trypsin is a porcine pancreas-derived enzyme that is commonly used for the dissociation and disaggregation of anchorage-dependent mammalian cells and tissues. The concentration of trypsin necessary to dislodge the cells from their substrate depends on the sensitivity of the cells.

HAT (Hypoxanthine, Aminopterin, Thymidine), Corning®

Catalog Number: (MDTC25-046-CI)

Supplier: Corning

Description: HAT Medium (hypoxanthine-aminopterin-thymidine medium) is used in mammalian cell culture for post-fusion selection to eliminate certain types of myeloma cells

UOM: 1 * 1 items

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Serum-free/low-protein medium, 1X with L-glutamine and 1 g/L BSA, Corning®

Catalog Number: (MDTC40-101-CV)

Supplier: Corning

Description: Corning® cellgro® COMPLETE formulation is based on a proprietary combination of 1:1 DMEM/F12, RPMI and McCoy's 5A medium. This medium contains a mixture of selected trace elements and high molecular weight carbohydrates, extra vitamins, animal origin-free protein, and a small amount of high quality bovine serum albumin (1,0 g/L). The Corning cellgro COMPLETE supports superior growth and viability over long-term passages in hybridomas, along with suspension and adherent cell cultures.

UOM: 1 * 6 items

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Cell dissociation reagent, Corning® Cellstripper®

Catalog Number: (25-056-CI)

Supplier: Corning

Description: Cellstripper® is a non-enzymatic cell dissociation solution formulated with a proprietary mixture of chelators that gently dislodge adherent cells in tissue culture. It is an alternative to trypsin when used with either serum-free or serum-supplemented media. Because Cellstripper® is a gentler reagent than trypsin, cells can be exposed to Cellstripper for longer periods of time. Cellstripper® achieves superior results in comparison to enzymatic dissociation products.

UOM: 1 * 6 items

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VWR® Media Supplements for Dehydrated Culture Media

Supplier: VWR Chemicals

Description: The isolation of microorganisms often requires the use of specific supplements. The supplements presented here are available in vials and bottles, and the corresponding culture medium to use with them is indicated in the table below. Usually each vial is used to supplement 500 ml of media unless otherwise stated (complete information available on the specification sheets available on request).

Cell culture media, Ham's F10 (F-10 Nutrient Medium)

Supplier: BIOWEST

Description: Ham's F10 medium is intended to support the growth of Chinese Hamster Ovary cells under serum-free conditions and other mammalian cell types with serum supplementation. It is a popular medium for growth of fastidious cell lines.

Cell culture media, Ham's F12 (F-12 Nutrient Medium)

Supplier: BIOWEST

Description: Ham's F12 was originally developed for the serum-free clonal growth of Chinese Hamster Ovary (CHO) cells, lung cells and mouse L-cells. It is frequently used with dialysed serum, hormones, selenium and other supplements for serum-free cultures. It is the medium of choice for supporting the growth of cells of rodent origin, particularly rabbit and rat and has proved to be an excellent cloning medium for the culture of myeloma and hybrid cells.

Cell culture media, McCoy's 5A medium

Supplier: BIOWEST

Description: McCoy’s media were originally formulated for growth and support of lymphocytes. This final modification produced a medium identical to RPMI 1629. McCoy's 5A media supports the indefinite proliferation of Walker 256 carcinoma cells. In addition, it is excellent for the propagation of leukocytes, biopsy tissues, a broad range of human and rat normal or transformed cell types, the most current primary and continuous cell lines.

Cell culture media, Liebovitz L-15 medium

Supplier: BIOWEST

Description: The Leibovitz L-15 media were formulated to promote the cell growth in medium not balanced in CO₂. The formulations were developed with the sodium bicarbonate buffer. The Leibovitz L-15 media are buffered by a complement of salts, free base amino acids and galactose so they can be used under conditions of free gaseous exchange with the atmosphere. When properly supplemented, L-15 Medium supports established cell lines, such as HEp-2 and LLC-MK2, as well as primary explants of embryonic and adult human tissue. Many viruses also have been successfully cultivated in this medium.

VWR® Cell Culture Dishes

Supplier: VWR Collection

Description: VWR® cell and tissue culture dishes are available with a choice of surface treatments.

Cell culture media, Medium 199

Supplier: BIOWEST

Description: The long term cultivation of cells requires the addition of serum. When properly supplemented, Medium 199 has broad species applicability, particularly for cultivation of non-transformed cells. It is widely used in virology, vaccine production and<i> in vitro</i> cultivation of primary explants of mouse pancreatic epithelial and rat lens tissues.

Cell culture media, RPMI 1640

Supplier: BIOWEST

Description: RPMI are general purpose enriched media with extensive applications for a broad spectrum of mammalian and hybridoma cells, including human myeloma, mouse hybridoma, human leukocytes, and B and T lymphocytes. It was originally formulated for suspension cultures and monolayer culture of human leukaemia cells.