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Catalog Number: (M307-1ML)
Supplier: VWR Chemicals
Description: Contains a mix of protease inhibitors: AEBSF, E-64, Bestatin, Pepstatin, Leupeptin and 1, 10-Phenanthroline. Each vial can be reconstituted in 1 ml dimethyl sulfoxide to form a 100X solution.
UOM: 1 * 1 mL


Supplier: BIOWEST
Description: RPMI are general purpose enriched media with extensive applications for a broad spectrum of mammalian and hybridoma cells, including human myeloma, mouse hybridoma, human leukocytes, and B and T lymphocytes. It was originally formulated for suspension cultures and monolayer culture of human leukaemia cells.
Supplier: BIOWEST
Description: The Leibovitz L-15 media were formulated to promote the cell growth in medium not balanced in CO₂. The formulations were developed with the sodium bicarbonate buffer. The Leibovitz L-15 media are buffered by a complement of salts, free base amino acids and galactose so they can be used under conditions of free gaseous exchange with the atmosphere. When properly supplemented, L-15 Medium supports established cell lines, such as HEp-2 and LLC-MK2, as well as primary explants of embryonic and adult human tissue. Many viruses also have been successfully cultivated in this medium.
Supplier: BIOWEST
Description: CRML was originally formulated for promoting strain L (Earle) cells under serum-free conditions. These media are also known for its ability to sustain cloning of monkey kidney cells and for growth of many other mammalian cell lines when supplemented with horse or calf serum.
Supplier: BIOWEST
Description: Ham's F12 was originally developed for the serum-free clonal growth of Chinese Hamster Ovary (CHO) cells, lung cells and mouse L-cells. It is frequently used with dialysed serum, hormones, selenium and other supplements for serum-free cultures. It is the medium of choice for supporting the growth of cells of rodent origin, particularly rabbit and rat and has proved to be an excellent cloning medium for the culture of myeloma and hybrid cells.
Supplier: BIOWEST
Description: The long term cultivation of cells requires the addition of serum. When properly supplemented, Medium 199 has broad species applicability, particularly for cultivation of non-transformed cells. It is widely used in virology, vaccine production and<i> in vitro</i> cultivation of primary explants of mouse pancreatic epithelial and rat lens tissues.
Catalog Number: (P5030-500GR)
Supplier: BIOWEST
Description: D-glucose monohydrate (Dextrose) is a common natural sugar involved in processes such as energy production, glycosylation, and formation of glycans that provide structure to cells. It is involved in a detrimental process in cells called glycation. It is used as a supplement for cell culture and in numerous cellular processes.
UOM: 1 * 500 g

Catalog Number: (392-0284)
Supplier: Greiner Bio-One
Description: PS, cell culture-treated, sterile. Cell culture treated plates are available with round (U), conical (V) or flat (F) bottom wells. All plates are certified free of RNase, DNase and human DNA, are non-pyrogenic and non-cytotoxic.
UOM: 1 * 60 items

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Catalog Number: (L0138-500)
Supplier: BIOWEST
Description: Ham's F14 medium was developed from Ham's F12 medium, which was originally developed for the serum-free clonal growth of Chinese Hamster Ovary (CHO) cells, lung cells and mouse L-cells. Ham's F14 contains a double concentration of amino acids compared to the Ham's F12 medium. This product is also supplemented with calcium chloride and ascorbic acid.
UOM: 1 * 500 mL

Supplier: BIOWEST
Description: McCoy’s media were originally formulated for growth and support of lymphocytes. This final modification produced a medium identical to RPMI 1629. McCoy's 5A media supports the indefinite proliferation of Walker 256 carcinoma cells. In addition, it is excellent for the propagation of leukocytes, biopsy tissues, a broad range of human and rat normal or transformed cell types, the most current primary and continuous cell lines.
Supplier: VWR Collection
Description: PS, PC or PETG, with PP screw cap, sterile.

Supplier: BIOWEST
Description: Studies to determine the nutritional requirements of many cells have been in progress since Eagle's first reports. The major essential nutrients were identified, and work became focussed on the media requirements of individual cell types. Many media designed for these purposes are now available. Among the first of these media, developed initially to study hormonal requirements of cells in culture, was a mixture of DMEM and Ham's F12 medium, known as DMEM-Ham's F12.
Supplier: BIOWEST
Description: Dulbecco's Modified Eagle Medium (DMEM) is a modification of Basal Medium Eagle (BME) which contains approximately four times as much of the vitamins and amino acids, and two to four times as much glucose (depending on the version) as the original formulation.
Supplier: BIOWEST
Description: These irrigating buffers and salt solutions are sterile, physiological balanced solutions intended for use in the maintenance of mammalian cells, where a chemically defined, balanced salt solution provides an environment that will maintain the structural and physiological integrity of cells <i>in vitro</i>. Applications include the intermediate steps for cultivation of cells, like washing, centrifugation, suspending and counting, as well as for many analytical methods or biochemical treatments. Principally these solutions should be used to keep the cells in suspension only for minutes or a few hours maximum. These buffers and solutions are not cell culture media.
Supplier: VWR Collection
Description: These square/octagonal PETG ergonomic media bottles are ideal for storage of media, laboratory reagents and biological buffers.

Catalog Number: (L0042-500)
Supplier: BIOWEST
Description: Basal Medium Eagle is principally used for monolayer diploid or primary cell cultures. It is the simplest of the basal media with all essential components for growth. BME ideally supports cell lines such as HeLa, L-cells and primary mammalian fibroblasts. BME has also successfully been used for the growth of human transformed cell lines (particularly WI-38 and MRC-5).
UOM: 1 * 500 mL

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Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us at 1-800-932-5000.
This product is marked as restricted and can only be purchased by approved Shipping Accounts. If you need further assistance, email VWR Regulatory Department at eurega_services@eu.vwr.com
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This product is no longer available. Alternatives may be available by searching with the VWR Catalog Number listed above. If you need further assistance, please call VWR Customer Service at 1-800-932-5000.
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