You Searched For: 5,5\'-Dithiobis(2-nitrobenzoic+acid)+(Ellmans+reagent,+DTNB)

Supplier: OMEGA BIOTEK

Description: E.Z.N.A.® MicroElute Total RNA Kit provides a rapid and easy method for the isolation of up to 50 µg of total RNA from small amounts of cultured eukaryotic cells, tissues such as as laser dissected samples (LDS) or fine needle aspirates(FNA). Normally, up to 5×10⁵ eukaryotic cells or 5 mg tissue can be used in a single experiment depending on the type of tissue used. This kit allows processing of single or multiple of samples in less than 30 minutes. There is no need for phenol/chloroform extractions, and time-consuming steps such as CsCl gradient ultracentrifuation, and precipitation with isopropanol or LiCl. Purified RNA can be eluted with 10 to 15 µl nuclease-free water. Purified RNA is ready for most downstream applications such as RT-PCR, Northern blotting, Poly A+ purification, nuclease protection and<i> invitro</i> translation.

MSDS Certificates

Supplier: OMEGA BIOTEK

Description: The E.Z.N.A.® tissue DNA kit offers a simple, rapid, and cost effective method for the isolation of DNA from a wide variety of sample sources including fresh or frozen animal cells and tissues. After cell lysis, the DNA purification process can be completed in less than 30 minutes. Up to 30 mg of tissue at a time can be readily processed at a time using the simple E.Z.N.A.® tissue DNA protocol. Single or multiple samples can be simultaneously processed with this spin-column based kit. There is no need for phenol/chloroform extractions, or time-consuming steps such as precipitation with isopropanol or ethanol. DNA purified using the E.Z.N.A.® tissue DNA kit is ready for most downstream applications such as PCR, southern blot and restriction enzyme digestion.

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Supplier: VWR Chemicals

Description: L(+)-Ascorbic acid free acid ≥99% ACS

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Supplier: RANKEM

Description: High purity analytical reagent.

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Catalog Number: (N788-KIT)

Supplier: VWR Chemicals

Description: The phenol-free total RNA purification kit provides a rapid method for the isolation and purification of total RNA from cultured animal cells, tissue samples, blood, bacteria, yeast, fungi, plants and viruses.

UOM: 1 * 1 KIT

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Supplier: RANKEM

Description: High purity analytical reagent that meets or exceeds the ACS reagent specifications.

MSDS Certificates

Catalog Number: (E530-1L)

Supplier: VWR Chemicals

Description: Bradford's reagent, Biotechnology Grade for quantitative protein determination, for quantitative protein determination

UOM: 1 * 1 L

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Catalog Number: (85976.290)

Supplier: VWR Chemicals

Description: Thioacetamide reagent I + II Reag. Ph. Eur. 1089601

UOM: 1 * 1 L

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Catalog Number: (220947S)

Supplier: VWR Chemicals

Description: Biuret reagent solution for protein (total) in serum or plasma

UOM: 1 * 5 L

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Catalog Number: (F781-05)

Supplier: Avantor

Description: Dithiothreitol (DTT, Cleland's reagent) ≥99.0%, Biotech Reagent, J.T.Baker®

UOM: 1 * 1 kg

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Supplier: RANKEM

Description: High purity analytical reagent

MSDS

Catalog Number: (20121.366)

Supplier: VWR Chemicals

Description: Acetic acid 12%

UOM: 1 * 5 L

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Supplier: OMEGA BIOTEK

Description: The Mag-Bind® PX blood RNA kit allows for the isolation of total RNA from 2,5 ml of whole blood that has been stored in PAXgene® Blood RNA tubes. Stabilised blood samples are first spun down and the nucleic acid pellets are washed and recollected. After washing, the nucleic acid pellet is dissolved into a specially formulated resuspension buffer. At this point the samples can be transferred to 96-well plates for processing in robotic liquid handlers and magnetic processors. High quality intact RNA can be isolated in less than 90 minutes.

Catalog Number: (D5525-01)

Supplier: OMEGA BIOTEK

Description: The E.Z.N.A.® Water DNA kit is formulated to isolate high purity cellular DNA from water samples typically containing humic acid and inhibitors of PCR. This kit has been successfully used to isolate DNA from tough-to-lyse bacteria, fungi, and algae that inhabit a range of samples including tap water, lake, river and sewage samples. Isolated DNA can be used for most downstream applications including PCR, Southern blotting, SNP analysis.

UOM: 1 * 50 Tests

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Catalog Number: (20308.156)

Supplier: VWR Chemicals

Description: EGTA (ethylene glycol bis(2-aminoethyl ether)-N,N,N',N'-tetraacetic acid) ≥98.5% analytical reagent

UOM: 1 * 50 g

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Supplier: OMEGA BIOTEK

Description: Plasmid isolated with traditional purification procedures normally contain high levels of endotoxins (also known as lipopolysaccharides or LPS) that can significantly interfere with transfection experiments downstream. The E.Z.N.A.® Endo-Free plasmid mini kit II integrates an efficient endotoxin removal step into the plasmid purification procedure to produce high-quality transfection grade (<0,1 EU/µg) plasmid for efficient transfection. The bacterial cells are lysed using the alkaline-SDS lysis method. The cleared cell lysate is then treated with ETR reagent to efficiently remove the endotoxins. After adjusting the binding condition, the cell lysate is applied into the HiBind® DNA column and purified DNA is eluted from the column membrane.

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