HEPES (2-[4-(2-hydroxyethyl)-1-piperazinyl]ethanesulphonic acid) free acid ≥99%, high purity

Supplier: VWR Chemicals

Synonyms: HEPES free acid, N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulphonic acid), 4-(2-Hydroxyethyl)piperazin-1-ylethanesulphonic acid, N-2-Hydroxyethylpiperazine-N′-2-ethanesulphonic acid, 2-(4-(2-Hydroxyethyl)piperazin-1-yl)ethanesulphonic acid

0511-250GEA 5636 INR
0511-250G 0511-50G
HEPES (2-[4-(2-hydroxyethyl)-1-piperazinyl]ethanesulphonic acid) free acid ≥99%, high purity
HEPES (4-(2-Hydroxyethyl)piperazin-1-ylethanesulphonic acid)

HEPES is a general purpose buffer available for biological research.


Has been used to advantage in tissue culture, oxidative phosphorylation, protein synthesis with cell-free bacterial systems, photophosphorylation and CO₂ fixation. Suitable buffer for TEM studies (Transmission Electron Microscopy) as it does not affect metal substrates. Recommended buffer for glutamate binding assays, preventing binding to non receptor materials.

Formula: C₈H₁₈N₂O₄S
MW: 238,31 g/mol
Melting Pt: 234 °C
Density: 1,448 g/cm³ (20 °C)
Storage Temperature: Ambient
MDL Number: MFCD00006158
CAS Number: 7365-45-9
EINECS: 230-907-9
Merck Index: 13,04672
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Specification Test Results

DNase none detected
Heavy Metals (as Pb) ≤0.0005%
Iron ≤0.0005%
pKa @20 °C 7.45 – 7.65
Protease none detected
Purity (Dry Basis) >99%
Residue on Ignition <0.2%
RNase none detected

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