Tris(hydroxymethyl)aminomethane (TRIS, Trometamol) ≥99.9%, Ultrapure
Supplier: VWR Chemicals
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Warning
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Synonyms:
2-Amino-2-(hydroxymethyl)propane-1,3-diol(tris), Tromethamine, Tris(hydroxymethyl)methylamine, 2-Amino-2-(hydroxymethyl)propane-1,3-diol, 2-Amino-2-(hydroxymethyl)-1,3-propanediol, Trizma(R) base, TRIS base, THAM
Reagent for stabilisation of buffer formulations and electrophoresis of biological molecules.
Biological buffers are useful for cell culture in vitro, enzyme assays and some electrophoretic applications at physiological pH. Universally applicable buffers for biochemistry must be water soluble, not interfere with biological processes or biological membranes (penetration, solubilisation, adsorption on surface, etc.), have known complex forming tendency with metal ions, be non toxic and have a very low U.V. absorption at wavelength >260 nm.
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Formula:
NH₂C(CH₂OH)₃ MW: 121,14 g/mol Boiling Pt: 288 °C Melting Pt: 169 °C Density: 1,328 g/cm³ (20 °C) Storage Temperature: Ambient |
MDL Number:
MFCD00004679 CAS Number: 77-86-1 EINECS: 201-064-4 Merck Index: 13,09842 |
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Specification Test Results
| Abs.@280 nm (1 M, Water) | <0.05 |
| Abs.@290 nm (40%, Water) | ≤0.2 |
| Abs.@400 nm (40%, Water) | ≤0.05 |
| Arsenic | ≤0.0005% |
| Copper | ≤0.0001% |
| Insolubles | ≤0.005% |
| Iron | ≤0.0001% |
| Lead | ≤0.0001% |
| Magnesium | ≤0.0005% |
| Melting Point | 168 – 172 °C |
| Moisture (KF) | ≤1% |
| pH (5%, Water) @25 °C | 10 - 11.5 |
| Purity | ≥99.9% |
| Solubility (10%, Water) | Pass |
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