HEPES (2-[4-(2-hydroxyethyl)-1-piperazinyl]ethanesulphonic acid), anhydrous ≥99.5% for biopharmaceutical production
Supplier: VWR Chemicals
Synonyms:
HEPES free acid, N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulphonic acid), 4-(2-Hydroxyethyl)piperazin-1-ylethanesulphonic acid, N-2-Hydroxyethylpiperazine-N′-2-ethanesulphonic acid, 2-(4-(2-Hydroxyethyl)piperazin-1-yl)ethanesulphonic acid
30487.297EA
14081
INR
30487.297
30487.366
30487.468
HEPES (2-[4-(2-hydroxyethyl)-1-piperazinyl]ethanesulphonic acid), anhydrous ≥99.5% for biopharmaceutical production
HEPES (4-(2-Hydroxyethyl)piperazin-1-ylethanesulphonic acid)
HEPES
Formula:
C₈H₁₈N₂O₄S MW: 238,31 g/mol Melting Pt: 210…215 °C Density: 1,448 g/cm³ (20 °C) Storage Temperature: Ambient |
MDL Number:
MFCD00006158 CAS Number: 7365-45-9 EINECS: 230-907-9 Merck Index: 13,04672 |
Specification Test Results
Assay | Min. 99.5 % |
Appearance | White crystalline powder |
Cell culture toxicity | Passes test |
DNases | Not detected |
Identification by IR | Conforms to structure |
Proteases | Not detected |
RNases | Not detected |
Solubility (1 mol/l) | Clear.colourless.complete |
pKa (20°C) | 7.45 - 7.65 |
Heavy metals (as Pb) | Max. 5 ppm |
Ignition residue | Max. 0.3 % |
Water | Max. 0.5 % |
Ca (Calcium) | Max. 5 ppm |
Cu (Copper) | Max. 5 ppm |
Fe (Iron) | Max. 5 ppm |
Mg (Magnesium) | Max. 5 ppm |
Mn (Manganese) | Max. 5 ppm |
Absorbance (260 nm) (1 mol/l) | Max. 0.10 |
Absorbance (280 nm) (1 mol/l) | Max. 0.10 |
Bioburden | Max. 100 CFU/g |
Endotoxin (0,2 %) | Max. 0.1 EU/mg |
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